BRENDA - Enzyme Database
show all sequences of 2.6.1.4

Glutamic-glycine transaminase from rat liver

Nakada, H.I.; J. Biol. Chem. 239, 468-471 (1964)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
50% inhibition at 5 mM
Rattus norvegicus
Isonicotinic acid hydrazide
glyoxylate partially protects
Rattus norvegicus
Maleic acid
weak
Rattus norvegicus
PCMB
no inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.6
-
glutamate
pH 7.3
Rattus norvegicus
8.3
-
glyoxylate
pH 7.3
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glyoxylate + L-glutamate
Rattus norvegicus
-
glycine + 2-oxoglutarate
-
Rattus norvegicus
ir
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial, about 30fold
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
limited activity
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
additional information
tissue distribution, very low activity in lung, testes or diaphragm
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.13
-
partially purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glyoxylate + L-alanine
no activity
639986
Rattus norvegicus
glycine + pyruvate
-
-
-
?
glyoxylate + L-glutamate
-
639986
Rattus norvegicus
glycine + 2-oxoglutarate
-
639986
Rattus norvegicus
ir
additional information
substrate specificity
639986
Rattus norvegicus
?
-
-
-
?
additional information
no substrates are D-amino acid isomers
639986
Rattus norvegicus
?
-
-
-
?
additional information
no activity with ammonia, glutathione, L-ornithine or 4-aminobutanoate
639986
Rattus norvegicus
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
glutamic-glycine transaminase
-
Rattus norvegicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
5 min, stable
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
8.2
about half-maximal activity at pH 6.2 and pH 8.2
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
addition to enzyme system mostly without effect
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
addition to enzyme system mostly without effect
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
50% inhibition at 5 mM
Rattus norvegicus
Isonicotinic acid hydrazide
glyoxylate partially protects
Rattus norvegicus
Maleic acid
weak
Rattus norvegicus
PCMB
no inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.6
-
glutamate
pH 7.3
Rattus norvegicus
8.3
-
glyoxylate
pH 7.3
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glyoxylate + L-glutamate
Rattus norvegicus
-
glycine + 2-oxoglutarate
-
Rattus norvegicus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
partial, about 30fold
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
limited activity
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
additional information
tissue distribution, very low activity in lung, testes or diaphragm
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.13
-
partially purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glyoxylate + L-alanine
no activity
639986
Rattus norvegicus
glycine + pyruvate
-
-
-
?
glyoxylate + L-glutamate
-
639986
Rattus norvegicus
glycine + 2-oxoglutarate
-
639986
Rattus norvegicus
ir
additional information
substrate specificity
639986
Rattus norvegicus
?
-
-
-
?
additional information
no substrates are D-amino acid isomers
639986
Rattus norvegicus
?
-
-
-
?
additional information
no activity with ammonia, glutathione, L-ornithine or 4-aminobutanoate
639986
Rattus norvegicus
?
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
5 min, stable
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
8.2
about half-maximal activity at pH 6.2 and pH 8.2
Rattus norvegicus
Other publictions for EC 2.6.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738913
Zhang
Distinct photorespiratory reac ...
Oryza sativa Japonica Group
J. Photochem. Photobiol. B
142
110-117
2015
-
-
1
-
-
-
-
6
-
-
2
-
-
1
-
-
-
-
-
1
-
-
4
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
1
-
-
4
1
-
-
-
3
-
-
-
-
-
1
1
-
-
-
703680
Kameya
Purification of three aminotra ...
Hydrogenobacter thermophilus
FEBS J.
277
1876-1885
2010
-
-
-
-
-
-
-
2
-
-
2
-
-
7
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
731270
Donini
Recombinant production of eigh ...
Homo sapiens
Biochem. J.
422
265-272
2009
-
-
2
-
-
-
-
3
2
-
1
-
-
3
-
-
2
-
-
-
4
-
9
-
2
4
-
-
-
2
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
3
2
-
1
-
-
-
-
2
-
-
4
-
9
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
639991
Yamaguchi
Some properties of glycine ami ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris No. 7
Biosci. Biotechnol. Biochem.
67
783-789
2003
-
-
-
-
-
-
4
2
-
-
2
2
-
5
-
-
1
-
-
1
1
1
4
1
1
1
-
1
2
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
5
-
2
-
-
2
2
-
-
-
1
-
1
1
1
4
1
1
-
1
2
1
1
1
-
-
-
-
-
-
-
636731
Orzechowski
Alanine aminotransferase and g ...
Zea mays
Acta Biochim. Pol.
46
447-457
1999
-
-
-
-
-
-
6
-
-
-
1
1
-
5
-
-
1
-
-
1
-
-
3
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
1
1
-
-
-
1
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639989
Galas
L-glutamate-glyoxylate aminotr ...
Lactiplantibacillus plantarum
Acta Microbiol. Pol.
7
243-252
1975
-
-
-
-
-
-
-
2
-
1
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639988
Rehfeld
Aminotransferases in peroxisom ...
Spinacia oleracea
J. Biol. Chem.
247
4803-4811
1972
-
-
-
-
-
-
1
2
2
-
-
1
-
1
-
-
-
-
-
1
1
-
2
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
-
-
1
-
-
-
-
-
1
1
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
639987
Thompson
-
Isolation and characterization ...
Homo sapiens
Arch. Biochem. Biophys.
117
599-603
1966
-
-
-
-
-
-
7
2
-
-
-
1
-
1
-
-
1
-
-
1
1
-
7
-
1
1
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
7
-
2
-
-
-
1
-
-
-
1
-
1
1
-
7
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
639986
Nakada
Glutamic-glycine transaminase ...
Rattus norvegicus
J. Biol. Chem.
239
468-471
1964
-
-
-
-
-
-
4
2
-
-
-
1
-
3
-
-
1
-
-
6
1
-
5
-
1
-
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
-
1
-
-
-
1
-
6
1
-
5
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-