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Literature summary for 2.6.1.39 extracted from

  • Miyazaki, T.; Miyazaki, J.; Yamane, H.; Nishiyama, M.
    alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus (2004), Microbiology, 150, 2327-2334.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
additional information enzyme disruption mutant, needs longer lag phase for growth and shows slower growth in minimal medium. Addition of alpha-aminoadipate or lysine shortens the lag phase and improves growth rate Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0133
-
2-oxoisovalerate pH 7.5, 45°C Thermus thermophilus
0.024
-
2-oxoadipate pH 7.5, 45°C Thermus thermophilus
0.0288
-
2-oxoisocaproate pH 7.5, 45°C Thermus thermophilus
0.1483
-
2-oxo-3-methylvalerate pH 7.5, 45°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
sedimentation equilibrium analysis, equilibrium constant 2.4 + 10-5 M Thermus thermophilus
43845
-
2 * 43845, calculated, 2 * 44000, SDS-PAGE Thermus thermophilus
44000
-
2 * 43845, calculated, 2 * 44000, SDS-PAGE Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
513
-
substrate phenylpyruvate, pH 7.5, 45°C Thermus thermophilus
561
-
substrate 2-oxo-3-methylvalerate, pH 7.5, 45°C Thermus thermophilus
568
-
substrate pyruvate, pH 7.5, 45°C Thermus thermophilus
661
-
substrate oxaloacetate, pH 7.5, 45°C Thermus thermophilus
1102
-
substrate 2-oxoisovalerate, pH 7.5, 45°C Thermus thermophilus
5079
-
substrate 2-oxoisocaproate, pH 7.5, 45°C Thermus thermophilus
6788
-
substrate 2-oxoadipate, pH 7.5, 45°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylvalerate + L-glutamate
-
Thermus thermophilus 2-amino-3-methylpentanoate + 2-oxoglutarate
-
?
2-oxoadipate + L-glutamate
-
Thermus thermophilus L-2-aminoadipate + 2-oxoglutarate
-
?
2-oxoisocaproate + L-glutamate
-
Thermus thermophilus L-leucine + 2-oxoglutarate
-
?
2-oxoisovalerate + L-glutamate
-
Thermus thermophilus L-valine + 2-oxoglutarate
-
?
oxaloacetate + L-glutamate
-
Thermus thermophilus 2-aminosuccinate + 2-oxoglutarate
-
?
phenylpyruvate + 2-aminoadipate
-
Thermus thermophilus L-phenylalanine + 2-oxoglutarate
-
?
pyruvate + L-glutamate
-
Thermus thermophilus L-alanine + 2-oxoglutarate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43845, calculated, 2 * 44000, SDS-PAGE Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
2-oxoisovalerate pH 7.5, 45°C Thermus thermophilus
3.5
-
2-oxo-3-methylvalerate pH 7.5, 45°C Thermus thermophilus
17.8
-
2-oxoisocaproate pH 7.5, 45°C Thermus thermophilus
22.3
-
2-oxoadipate pH 7.5, 45°C Thermus thermophilus