Cloned (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme disruption mutant, needs longer lag phase for growth and shows slower growth in minimal medium. Addition of alpha-aminoadipate or lysine shortens the lag phase and improves growth rate | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0133 | - |
2-oxoisovalerate | pH 7.5, 45°C | Thermus thermophilus | |
0.024 | - |
2-oxoadipate | pH 7.5, 45°C | Thermus thermophilus | |
0.0288 | - |
2-oxoisocaproate | pH 7.5, 45°C | Thermus thermophilus | |
0.1483 | - |
2-oxo-3-methylvalerate | pH 7.5, 45°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
sedimentation equilibrium analysis, equilibrium constant 2.4 + 10-5 M | Thermus thermophilus |
43845 | - |
2 * 43845, calculated, 2 * 44000, SDS-PAGE | Thermus thermophilus |
44000 | - |
2 * 43845, calculated, 2 * 44000, SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
513 | - |
substrate phenylpyruvate, pH 7.5, 45°C | Thermus thermophilus |
561 | - |
substrate 2-oxo-3-methylvalerate, pH 7.5, 45°C | Thermus thermophilus |
568 | - |
substrate pyruvate, pH 7.5, 45°C | Thermus thermophilus |
661 | - |
substrate oxaloacetate, pH 7.5, 45°C | Thermus thermophilus |
1102 | - |
substrate 2-oxoisovalerate, pH 7.5, 45°C | Thermus thermophilus |
5079 | - |
substrate 2-oxoisocaproate, pH 7.5, 45°C | Thermus thermophilus |
6788 | - |
substrate 2-oxoadipate, pH 7.5, 45°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylvalerate + L-glutamate | - |
Thermus thermophilus | 2-amino-3-methylpentanoate + 2-oxoglutarate | - |
? | |
2-oxoadipate + L-glutamate | - |
Thermus thermophilus | L-2-aminoadipate + 2-oxoglutarate | - |
? | |
2-oxoisocaproate + L-glutamate | - |
Thermus thermophilus | L-leucine + 2-oxoglutarate | - |
? | |
2-oxoisovalerate + L-glutamate | - |
Thermus thermophilus | L-valine + 2-oxoglutarate | - |
? | |
oxaloacetate + L-glutamate | - |
Thermus thermophilus | 2-aminosuccinate + 2-oxoglutarate | - |
? | |
phenylpyruvate + 2-aminoadipate | - |
Thermus thermophilus | L-phenylalanine + 2-oxoglutarate | - |
? | |
pyruvate + L-glutamate | - |
Thermus thermophilus | L-alanine + 2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43845, calculated, 2 * 44000, SDS-PAGE | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
2-oxoisovalerate | pH 7.5, 45°C | Thermus thermophilus | |
3.5 | - |
2-oxo-3-methylvalerate | pH 7.5, 45°C | Thermus thermophilus | |
17.8 | - |
2-oxoisocaproate | pH 7.5, 45°C | Thermus thermophilus | |
22.3 | - |
2-oxoadipate | pH 7.5, 45°C | Thermus thermophilus |