KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
pyruvate | pH 8.0, 37°C | Streptomyces tendae | |
25 | - |
L-histidine | pH 8.0, 37°C | Streptomyces tendae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Streptomyces tendae |
85000 | - |
gel filtration | Streptomyces tendae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
imidazol-5-yl-pyruvate + L-glutamate | Streptomyces tendae | involved in nikkomycin biosynthesis, essential in the utilization of imidazol-L-lactate and imidazole pyruvate as carbon sources | histidine + 2-oxoglutarate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces tendae | - |
Tue901/AEC6, mutant hut-11 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces tendae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.866 | - |
- |
Streptomyces tendae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histidine + 2-oxocaproate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-norleucine | - |
r | |
histidine + 2-oxoglutarate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-glutamate | - |
r | |
histidine + 2-oxoisovalerate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-valine | - |
r | |
histidine + 2-oxovalerate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + L-norvaline | - |
r | |
imidazol-5-yl-pyruvate + L-glutamate | involved in nikkomycin biosynthesis, essential in the utilization of imidazol-L-lactate and imidazole pyruvate as carbon sources | Streptomyces tendae | histidine + 2-oxoglutarate | - |
r | |
L-histidine + 2-oxobutyrate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + 2-aminobutanoate | - |
r | |
L-histidine + glyoxylate | - |
Streptomyces tendae | imidazol-5-yl-pyruvate + glycine | - |
r | |
L-histidine + pyruvate | ping pong reaction mechanism | Streptomyces tendae | imidazol-5-yl-pyruvate + L-alanine | - |
r | |
additional information | does not use aromatic amino acids as amino substrates, L-phenylalanine, L-tyrosine, L-tryptophan and D-histidine are no substrates, does not react with L-histidinol phosphate, no activity on gels towards L-alanine, L-leucine, L-lysine, L-glutamate and L-aspartate with pyruvate as amino acceptor, no activity towards L-aspartate with 2-oxoglutarate | Streptomyces tendae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Streptomyces tendae |
Synonyms | Comment | Organism |
---|---|---|
HISAT | - |
Streptomyces tendae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptomyces tendae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptomyces tendae |