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Literature summary for 2.6.1.38 extracted from

  • Hacking, A.J.; Hassall, H.
    The purification and properties of L-histidine--2-oxoglutarate aminotransferase from Pseudomonas testosteroni (1975), Biochem. J., 147, 327-334.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.38

General Stability

General Stability Organism
purified preparations are unstable, activity is lost during purification, EDTA, DTT or 2-mercaptoethanol stabilizes during purification, pyridoxal 5'-phosphate restores activity Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
 Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 (imidazol-5-yl)pyruvate pH 8.0, 30°C Comamonas testosteroni
4
-
 L-histidine pH 8.0, 30°C Comamonas testosteroni
5
-
 L-glutamate pH 8.0, 30°C Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
imidazol-5-yl-pyruvate + L-glutamate Comamonas testosteroni essential for utilization of imidazolyl-L-lactate as sole carbon source histidine + 2-oxoglutarate
-
 r
imidazol-5-yl-pyruvate + L-glutamate Comamonas testosteroni N.C.I.B. 10808 essential for utilization of imidazolyl-L-lactate as sole carbon source histidine + 2-oxoglutarate
-
 r

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
 N.C.I.B. 10808
-
Comamonas testosteroni N.C.I.B. 10808
-
 N.C.I.B. 10808
-

Purification (Commentary)

Purification (Comment) Organism
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.8
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(imidazol-5-yl)pyruvate + L-glutamate
-
 Comamonas testosteroni L-histidine + 2-oxoglutarate
-
 r
(imidazol-5-yl)pyruvate + L-glutamate
-
 Comamonas testosteroni N.C.I.B. 10808 L-histidine + 2-oxoglutarate
-
 r
imidazol-5-yl-pyruvate + L-glutamate reaction mechanism ping pong, great specificity for histidine as amino donor, highly specific for 2-oxoglutarate in the reverse reaction, very slight activity with 2-oxomalonate or 4-methyl-2-oxopentanoate, L-tyrosine, phenylalanine or L-tryptophan Comamonas testosteroni L-histidine + 2-oxoglutarate
-
 r
imidazol-5-yl-pyruvate + L-glutamate reaction mechanism ping pong, great specificity for histidine as amino donor, highly specific for 2-oxoglutarate in the reverse reaction, very slight activity with 2-oxomalonate or 4-methyl-2-oxopentanoate, L-tyrosine, phenylalanine or L-tryptophan Comamonas testosteroni N.C.I.B. 10808 L-histidine + 2-oxoglutarate
-
 r
imidazol-5-yl-pyruvate + L-glutamate essential for utilization of imidazolyl-L-lactate as sole carbon source Comamonas testosteroni histidine + 2-oxoglutarate
-
 r
imidazol-5-yl-pyruvate + L-glutamate essential for utilization of imidazolyl-L-lactate as sole carbon source Comamonas testosteroni N.C.I.B. 10808 histidine + 2-oxoglutarate
-
 r
additional information L-histidinol phosphate, D-histidine, 5'-N-methyl-L-histidine and pyruvate are no substrates Comamonas testosteroni ?
-
 ?
additional information L-histidinol phosphate, D-histidine, 5'-N-methyl-L-histidine and pyruvate are no substrates Comamonas testosteroni N.C.I.B. 10808 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Comamonas testosteroni

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 optimal activity in this pH range, outside this range activity decreases sharply Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate inactive in absence, Km 0.000059 mM Comamonas testosteroni