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Literature summary for 2.6.1.37 extracted from

  • Panmanee, W.; Charoenlap, N.; Atichartpongkul, S.; Mahavihakanont, A.; Whiteside, M.D.; Winsor, G.; Brinkman, F.S.L.; Mongkolsuk, S.; Hassett, D.J.
    The OxyR-regulated phnW gene encoding 2-aminoethylphosphonate pyruvate aminotransferase helps protect Pseudomonas aeruginosa from tert-butyl hydroperoxide (2017), PLoS ONE, 12, e0189066 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.37

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa ATCC 15692
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa 1C
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa PRS 101
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa DSM 22644
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa CIP 104116
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa LMG 12228
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate Pseudomonas aeruginosa JCM 14847
-
 2-phosphonoacetaldehyde + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I434
-
-
Pseudomonas aeruginosa 1C Q9I434
-
-
Pseudomonas aeruginosa ATCC 15692 Q9I434
-
-
Pseudomonas aeruginosa CIP 104116 Q9I434
-
-
Pseudomonas aeruginosa DSM 22644 Q9I434
-
-
Pseudomonas aeruginosa JCM 14847 Q9I434
-
-
Pseudomonas aeruginosa LMG 12228 Q9I434
-
-
Pseudomonas aeruginosa PRS 101 Q9I434
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa ATCC 15692 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa 1C 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa PRS 101 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa DSM 22644 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa CIP 104116 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa LMG 12228 2-phosphonoacetaldehyde + L-alanine
-
 ?
(2-aminoethyl)phosphonate + pyruvate
-
 Pseudomonas aeruginosa JCM 14847 2-phosphonoacetaldehyde + L-alanine
-
 ?

Synonyms

Synonyms Comment Organism
2-aminoethylphosphonate:pyruvate aminotransferase
-
 Pseudomonas aeruginosa
PA1310
-
 Pseudomonas aeruginosa
phnW
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction gene phnW, encoding 2-aminoethylphosphonate:pyruvate aminotransferase, exhibits reduced expression in the oxyR mutant compared to wild-type bacteria. A phnW mutant is more susceptible to tert-butyl-hydroperoxide (t-BOOH) treatment than wild-type bacteria. This may be due to a reduced efficiency for this strain to degrade t-BOOH relative to wild-type organisms because of modulation of AHP gene transcription in the phnW mutant. Provision of the phnW gene in trans can also protect a strain lacking AhpC and Ohr proteins (ohr ahpC mutant), that have previously been shown to be highly susceptible to organic hydroperoxides including t-BOOH. The phnW mutant degrades only 55% of the t-BOOH compared to the 80% degradation observed in wild-type bacteria at the end of assay Pseudomonas aeruginosa
physiological function the OxyR-regulated phnW gene encoding enzyme 2-aminoethylphosphonate:pyruvate aminotransferase helps protect Pseudomonas aeruginosa from tert-butyl hydroperoxide. The LysR member of bacterial transactivators, OxyR, governs transcription of genes involved in the response to H2O2 and organic (alkyl) hydroperoxides (AHP) in the Gram-negative pathogen, Pseudomonas aeruginosa. Analysis of binding of OxyR to the phnW promoter, binding sequence determination, overview. The enzyme PhnW influences the degradation of tert-butyl-hydroperoxide (t-BOOH), but purified does not show a direct ability to degrade t-BOOH in vitro. Tert-butyl-hydroperoxide can cause apoptosis Pseudomonas aeruginosa