Application | Comment | Organism |
---|---|---|
nutrition | enzyme is involved in generation of mousy off-flavors in wine. Enzymic product 2-aminoadipate 6-semialdehyde is further transformed to 1-piperideine-6-carboxylic acid, which may react with methylglyoxal to generate the acylated N-heterocyclic odorant 2-acetyltetrahydropyridine | Streptomyces clavuligerus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Streptomyces clavuligerus |
expression in Escherichia coli | Streptomyces clavuligerus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48900 | - |
x * 49890, calculated, x * 48900, SDS-PAGE | Streptomyces clavuligerus |
49800 | - |
SDS-PAGE | Streptomyces clavuligerus |
49890 | - |
calculated from sequence of cDNA | Streptomyces clavuligerus |
49890 | - |
x * 49890, calculated, x * 48900, SDS-PAGE | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Streptomyces clavuligerus | Q5XPV2 | - |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S-200 gel filtration | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
pH 7.2, 37°C, wild-type enzyme | Streptomyces clavuligerus |
1.78 | - |
pH 7.2, 37°C, recombinant enzyme | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate | - |
Streptomyces clavuligerus | 2-aminoadipate 6-semialdehyde + ? | - |
? | |
L-lysine + 2-oxoglutarate | - |
Streptomyces clavuligerus | 2-aminoadipate 6-semialdehyde + 1-piperideine-6-carboxylic acid + L-glutamate | product 2-aminoadipate 6-semialdehyde is further transformed to 1-piperideine-6-carboxylic acid, which may react with methylglyoxal to generate the acylated N-heterocyclic odorant 2-acetyltetrahydropyridine | ? | |
additional information | the enzyme is responsible for the formation of 1-piperideine-6-carboxylic acid, which may react with methylglyoxal to generate the acylated N-heterocyclic odorant 2-acetyltetrahydropyridine | Streptomyces clavuligerus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49890, calculated, x * 48900, SDS-PAGE | Streptomyces clavuligerus |
Synonyms | Comment | Organism |
---|---|---|
L-lysine-epsilon-aminotransferase | - |
Streptomyces clavuligerus |
LAT | - |
Streptomyces clavuligerus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Streptomyces clavuligerus |
6.5 | 7.5 | broad | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Streptomyces clavuligerus |