Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.33 extracted from

  • Wang, Y.; Xu, Y.; Perepelov, A.V.; Qi, Y.; Knirel, Y.A.; Wang, L.; Feng, L.
    Biochemical characterization of dTDP-D-Qui4N and dTDP-D-Qui4NAc biosynthetic pathways in Shigella dysenteriae type 7 and Escherichia coli O7 (2007), J. Bacteriol., 189, 8626-8635.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expression with His-tag Escherichia coli
expression with His-tag Shigella dysenteriae

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 20% conversion in presence of Co2+ Escherichia coli
Co2+ 3.9% conversion in presence of Co2+ Shigella dysenteriae
Fe2+
-
 Escherichia coli
Fe2+
-
 Shigella dysenteriae
Mn2+
-
 Escherichia coli
Mn2+
-
 Shigella dysenteriae
additional information not inhibitory: Mg2+, EDTA Escherichia coli
additional information not inhibitory: Mg2+, EDTA Shigella dysenteriae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0458
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Escherichia coli
0.98
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Shigella dysenteriae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli O7
-
-
-
Shigella dysenteriae
-
 strain type 7
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-6-deoxy-D-glucose + glycine L-glycine, poor amino donor Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + glyoxylate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-alanine L-alanine, poor amino donor Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + pyruvate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-alanine L-alanine, poor amino donor Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + pyruvate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-alanine L-alanine, poor amino donor Escherichia coli O7 dTDP-4-amino-4,6-dideoxy-D-galactose + pyruvate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate co-substrate L-glutamate, 84.9% conversion rate Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate co-substrate L-glutamate, 85.5% conversion rate Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate co-substrate L-glutamate, 85.5% conversion rate Escherichia coli O7 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine co-substrate L-glutamine, 15.9% conversion rate Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutamine
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine co-substrate L-glutamine, 74.1% conversion rate Shigella dysenteriae dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutamine
-
 ?
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine co-substrate L-glutamine, 15.9% conversion rate Escherichia coli O7 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutamine
-
 ?

Synonyms

Synonyms Comment Organism
VioA
-
 Escherichia coli
VioA
-
 Shigella dysenteriae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Shigella dysenteriae
5.66
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.4, 37°C Escherichia coli