KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
TDP-4-amino-4,6-dideoxy-alpha-D-glucose | 38°C, pH 7.0-7.6 | Escherichia coli | |
0.14 | - |
TDP-4-keto-6-deoxy-D-glucose | 38°C, pH 7.0-7.6 | Escherichia coli | |
0.22 | - |
2-oxoglutarate | 38°C, pH 7.0-7.6 | Escherichia coli | |
5.1 | - |
L-glutamate | 38°C, pH 7.0-7.6 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | Escherichia coli | - |
dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate | - |
r | |
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | Escherichia coli B / ATCC 11303 | - |
dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial from strain B, 28fold | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.047 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0°C or -15°C, partially purified enzyme, stable for several weeks | Escherichia coli |
0°C, crude cell extract, stable for several days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate | - |
r | |
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | specific for the substrates | Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate | - |
r | |
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | - |
Escherichia coli B / ATCC 11303 | dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate | - |
r | |
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | specific for the substrates | Escherichia coli B / ATCC 11303 | dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate | - |
r | |
additional information | DL-glutamine, L-glutamine, D-glutamate, L-asparagine, DL-aspartate and L-alanine are inactive as amino donor, TDP-4-amino-4,6-dideoxy-D-galactose is inactive in the reverse reaction, neither pyruvate nor oxaloacetate can replace 2-oxoglutarate | Escherichia coli | ? | - |
? | |
additional information | DL-glutamine, L-glutamine, D-glutamate, L-asparagine, DL-aspartate and L-alanine are inactive as amino donor, TDP-4-amino-4,6-dideoxy-D-galactose is inactive in the reverse reaction, neither pyruvate nor oxaloacetate can replace 2-oxoglutarate | Escherichia coli B / ATCC 11303 | ? | - |
? | |
TDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate | - |
Escherichia coli | TDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate | - |
? | |
TDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate | - |
Escherichia coli B / ATCC 11303 | TDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | absolute requirement, Km is 0.00003 mM in reverse reaction, 0.00012 mM in forward reaction | Escherichia coli |