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Literature summary for 2.6.1.33 extracted from

  • Matsuhashi, M.; Strominger, J.L.
    Thymidine diphosphate 4-acetamido-2,6-dideoxyhexoses. 3. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Escherichia coli strain B (1966), J. Biol. Chem., 241, 4738-4744.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
TDP-4-amino-4,6-dideoxy-alpha-D-glucose 38°C, pH 7.0-7.6 Escherichia coli
0.14
-
TDP-4-keto-6-deoxy-D-glucose 38°C, pH 7.0-7.6 Escherichia coli
0.22
-
2-oxoglutarate 38°C, pH 7.0-7.6 Escherichia coli
5.1
-
L-glutamate 38°C, pH 7.0-7.6 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate Escherichia coli
-
dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate
-
r
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate Escherichia coli B / ATCC 11303
-
dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial from strain B, 28fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.047
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
0°C or -15°C, partially purified enzyme, stable for several weeks Escherichia coli
0°C, crude cell extract, stable for several days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
Escherichia coli dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate
-
r
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate specific for the substrates Escherichia coli dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate
-
r
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
Escherichia coli B / ATCC 11303 dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate
-
r
dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate specific for the substrates Escherichia coli B / ATCC 11303 dTDP-4-amino-4,6-dideoxy-D-glucose + 2-oxoglutarate
-
r
additional information DL-glutamine, L-glutamine, D-glutamate, L-asparagine, DL-aspartate and L-alanine are inactive as amino donor, TDP-4-amino-4,6-dideoxy-D-galactose is inactive in the reverse reaction, neither pyruvate nor oxaloacetate can replace 2-oxoglutarate Escherichia coli ?
-
?
additional information DL-glutamine, L-glutamine, D-glutamate, L-asparagine, DL-aspartate and L-alanine are inactive as amino donor, TDP-4-amino-4,6-dideoxy-D-galactose is inactive in the reverse reaction, neither pyruvate nor oxaloacetate can replace 2-oxoglutarate Escherichia coli B / ATCC 11303 ?
-
?
TDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate
-
Escherichia coli TDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate
-
?
TDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate
-
Escherichia coli B / ATCC 11303 TDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate absolute requirement, Km is 0.00003 mM in reverse reaction, 0.00012 mM in forward reaction Escherichia coli