Application | Comment | Organism |
---|---|---|
synthesis | pyridoxamine production by bioconversion is generally preferable for environmental and energetic aspects compared to chemical synthesis. Pyridoxamine is produced from pyridoxine, a readily and economically available starting material, by bioconversion using a Rhodococcus expression system | Mesorhizobium japonicum |
Cloned (Comment) | Organism |
---|---|
gene ppaT, functional recombinant expression of soluble enzyme in Rhodococcus erythropolis strain JCM3191, co-expression with pyridoxine 4-oxidase (PNO) from Mesorhizobium loti from gene pno | Mesorhizobium japonicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview | Mesorhizobium japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + pyruvate | Mesorhizobium japonicum | - |
pyridoxal + L-alanine | - |
? | |
pyridoxamine + pyruvate | Mesorhizobium japonicum LMG 29417 | - |
pyridoxal + L-alanine | - |
? | |
pyridoxamine + pyruvate | Mesorhizobium japonicum CECT 9101 | - |
pyridoxal + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium japonicum | Q988B8 | i.e. Mesorhizobium loti strain MAFF 303099 | - |
Mesorhizobium japonicum CECT 9101 | Q988B8 | i.e. Mesorhizobium loti strain MAFF 303099 | - |
Mesorhizobium japonicum LMG 29417 | Q988B8 | i.e. Mesorhizobium loti strain MAFF 303099 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine + pyruvate | - |
Mesorhizobium japonicum | pyridoxal + L-alanine | - |
? | |
pyridoxamine + pyruvate | - |
Mesorhizobium japonicum LMG 29417 | pyridoxal + L-alanine | - |
? | |
pyridoxamine + pyruvate | - |
Mesorhizobium japonicum CECT 9101 | pyridoxal + L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant soluble enzyme, SDS-PAGE | Mesorhizobium japonicum |
Synonyms | Comment | Organism |
---|---|---|
PPAT | - |
Mesorhizobium japonicum |
pyridoxamine-pyruvate aminotransferase | - |
Mesorhizobium japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | in vivo, recombinant enzyme in Rhodococcus erythropolis cells | Mesorhizobium japonicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | over 50% of maximal activity within this range | Mesorhizobium japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
in vivo, recombinant enzyme in Rhodococcus erythropolis cells | Mesorhizobium japonicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | over 50% of maximal activity within this range | Mesorhizobium japonicum |