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Literature summary for 2.6.1.29 extracted from

  • Grigoriou, S.; Kugler, P.; Kulcinskaja, E.; Walter, F.; King, J.; Hill, P.; Wendisch, V.F.; O'Reilly, E.
    Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions (2020), Green Chem., 22, 4128-4132 .
No PubMed abstract available

Application

Application Comment Organism
synthesis diamine donors are used for effective equilibrium displacement of transaminase-mediated biotransformations. Whole-cell biotransformation using engineered Corynebacterium glutamicum cells, that produce diamine donors, is evaluated Pseudomonas fluorescens
synthesis diamine donors are used for effective equilibrium displacement of transaminase-mediated biotransformations. Whole-cell biotransformation using engineered Corynebacterium glutamicum cells, that produce diamine donors, is evaluated Pseudomonas putida
synthesis diamine donors are used for effective equilibrium displacement of transaminase-mediated biotransformations. Whole-cell biotransformation using engineered Corynebacterium glutamicum cells, that produce diamine donors, is evaluated Pseudomonas chlororaphis subsp. aureofaciens
synthesis diamine donors are used for effective equilibrium displacement of transaminase-mediated biotransformations. Whole-cell biotransformation using engineered Corynebacterium glutamicum cells, that produce diamine donors, is evaluated Ruegeria pomeroyi

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Corynebacterium glutamicum Pseudomonas fluorescens
recombinant expression in Corynebacterium glutamicum Pseudomonas putida
recombinant expression in Corynebacterium glutamicum Pseudomonas chlororaphis subsp. aureofaciens
recombinant expression in Corynebacterium glutamicum Ruegeria pomeroyi

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis subsp. aureofaciens
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas putida
-
-
-
Ruegeria pomeroyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenylpentane-2,4-dione + cadaverine 43% conversion at 5 mM cadaverine, 80% at 15 mM cadaverine Pseudomonas chlororaphis subsp. aureofaciens ?
-
?
1-phenylpentane-2,4-dione + cadaverine 53% conversion at 5 mM cadaverine, 75% at 15 mM cadaverine Pseudomonas putida ?
-
?
1-phenylpentane-2,4-dione + cadaverine 54% conversion at 5 mM cadaverine, 78% at 15 mM cadaverine Pseudomonas fluorescens ?
-
?
1-phenylpentane-2,4-dione + cadaverine 75% conversion at 5 mM cadaverine, 99% at 15 mM cadaverine Ruegeria pomeroyi ?
-
?
1-phenylpentane-2,4-dione + o-xylylenediamine 25% conversion Pseudomonas putida ?
-
?
1-phenylpentane-2,4-dione + o-xylylenediamine 35% conversion Pseudomonas fluorescens ?
-
?
1-phenylpentane-2,4-dione + o-xylylenediamine 74% conversion Ruegeria pomeroyi ?
-
?
1-phenylpentane-2,4-dione + o-xylylenediamine 23% conversion Pseudomonas chlororaphis subsp. aureofaciens ?
-
?
1-phenylpentane-2,4-dione + putrescine 20% conversion Pseudomonas chlororaphis subsp. aureofaciens ?
-
?
1-phenylpentane-2,4-dione + putrescine 24% conversion Pseudomonas putida ?
-
?
1-phenylpentane-2,4-dione + putrescine 28% conversion Pseudomonas fluorescens ?
-
?
1-phenylpentane-2,4-dione + putrescine 72% conversion Ruegeria pomeroyi ?
-
?
4-phenylbutan-2-one + cadaverine 14% conversion Pseudomonas chlororaphis subsp. aureofaciens ?
-
?
4-phenylbutan-2-one + cadaverine 18% conversion Pseudomonas putida ?
-
?
4-phenylbutan-2-one + cadaverine 21% conversion Ruegeria pomeroyi ?
-
?
4-phenylbutan-2-one + cadaverine 25% conversion Pseudomonas fluorescens ?
-
?
4-phenylbutan-2-one + o-xylylenediamine 11% conversion Pseudomonas putida ?
-
?
4-phenylbutan-2-one + o-xylylenediamine 17% conversion Pseudomonas fluorescens ?
-
?
4-phenylbutan-2-one + o-xylylenediamine 20% conversion Ruegeria pomeroyi ?
-
?
4-phenylbutan-2-one + o-xylylenediamine 8% conversion Pseudomonas chlororaphis subsp. aureofaciens ?
-
?
4-phenylbutan-2-one + putrescine 6% conversion Pseudomonas chlororaphis subsp. aureofaciens ?
-
?
4-phenylbutan-2-one + putrescine 9% conversion Pseudomonas fluorescens ?
-
?
4-phenylbutan-2-one + putrescine 9% conversion Pseudomonas putida ?
-
?
4-phenylbutan-2-one + putrescine 19% conversion Ruegeria pomeroyi ?
-
?
benzaldehyde + cadaverine 9% conversion Pseudomonas fluorescens ?
-
?
benzaldehyde + o-xylylenediamine 7% conversion Pseudomonas fluorescens ?
-
?
additional information development of a whole-cell system for the co-production of an omega-TA and sacrificial smart amine donor. Optimization of reaction conditions, 5 mM amine substrate concentration. No activity with benzaldehyde as amine acceptor Pseudomonas putida ?
-
-
additional information development of a whole-cell system for the co-production of an omega-TA and sacrificial smart amine donor. Optimization of reaction conditions, 5 mM amine substrate concentration. No activity with benzaldehyde as amine acceptor Pseudomonas chlororaphis subsp. aureofaciens ?
-
-
additional information development of a whole-cell system for the co-production of an omega-TA and sacrificial smart amine donor. Optimization of reaction conditions, 5 mM amine substrate concentration. No activity with putrescine as amine donor and benzaldehyde as amine acceptor Pseudomonas fluorescens ?
-
-
additional information development of a whole-cell system for the co-production of an omega-transaminase and sacrificial smart amine donor. Optimization of reaction conditions, 5 mM amine substrate concentration. No activity with benzaldehyde as amine acceptor Ruegeria pomeroyi ?
-
-

Synonyms

Synonyms Comment Organism
(S)-selective ATA
-
Pseudomonas fluorescens
(S)-selective ATA
-
Pseudomonas putida
(S)-selective ATA
-
Pseudomonas chlororaphis subsp. aureofaciens
(S)-selective ATA
-
Ruegeria pomeroyi
3HMU
-
Ruegeria pomeroyi
omega-diamine aminotransferase
-
Pseudomonas fluorescens
omega-diamine aminotransferase
-
Pseudomonas putida
omega-diamine aminotransferase
-
Pseudomonas chlororaphis subsp. aureofaciens
omega-diamine aminotransferase
-
Ruegeria pomeroyi
PcATA
-
Pseudomonas chlororaphis subsp. aureofaciens
PfATA
-
Pseudomonas fluorescens
PpATA
-
Pseudomonas putida

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Pseudomonas fluorescens
30
-
assay at Pseudomonas putida
30
-
assay at Pseudomonas chlororaphis subsp. aureofaciens
30
-
assay at Ruegeria pomeroyi

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
the ATA retains less than 20% activity in the intracellular neutral pH Pseudomonas fluorescens
additional information
-
the ATA retains less than 20% activity in the intracellular neutral pH Pseudomonas putida
additional information
-
the ATA retains less than 20% activity in the intracellular neutral pH Pseudomonas chlororaphis subsp. aureofaciens
additional information
-
the ATA retains less than 20% activity in the intracellular neutral pH Ruegeria pomeroyi

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP Pseudomonas fluorescens
pyridoxal 5'-phosphate PLP Pseudomonas putida
pyridoxal 5'-phosphate PLP Pseudomonas chlororaphis subsp. aureofaciens
pyridoxal 5'-phosphate PLP Ruegeria pomeroyi