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Literature summary for 2.6.1.29 extracted from

  • Kim, K.H.; Tchen, T.T.
    Diamine-alpha-ketoglutarate aminotransferase (Escherichia coli) (1971), Methods Enzymol., 17B, 812-815.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
cyanide complete inhibition at 0.5 mM Escherichia coli
hydroxylamine complete inhibition at 0.5 mM Escherichia coli
Semicarbazide complete inhibition at 0.5 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
2-oxoglutarate pH 7.6, room temperature Escherichia coli
2.7
-
pyruvate pH 7.6, room temperature Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
putrescine + 2-oxoglutarate Escherichia coli
-
4-aminobutanal + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
precipitation with ammonium sulphate followed by column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
1.63
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-15┬║C, 0.03 M Tris-HCl buffer, pH 7.6, 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-1,4-diaminobutan-2-ol + 2-oxoglutarate 30% of the activity with putrescine Escherichia coli ?
-
?
1,6-diaminohexane + 2-oxoglutarate
-
Escherichia coli 6-aminohexanal + L-glutamate
-
?
1,7-diaminoheptane + 2-oxoglutarate 30% of the activity with putrescine Escherichia coli 7-aminoheptanal + L-glutamate
-
?
4-aminobutanoate + 2-oxoglutarate
-
Escherichia coli 4-oxobutanoate + L-glutamate
-
?
cadaverine + 2-oxoglutarate equally active as putrescine Escherichia coli 5-aminopentanal + L-glutamate
-
?
additional information inactive with 1,3-diaminopropane, lysine, ornithine, spermidine Escherichia coli ?
-
?
additional information inactive with histamine, tyramine, 4-aminobutanol, beta-alanine Escherichia coli ?
-
?
putrescine + 2-oxoglutarate
-
Escherichia coli 4-aminobutanal + L-glutamate
-
?
putrescine + pyruvate
-
Escherichia coli 4-aminobutanal + L-alanine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate purified enzyme normally active without added pyridoxal phosphate, absolute requirement when irradiated with UV light or treated with 2 mM phenylhydrazine and then with Norite Escherichia coli