General Stability | Organism |
---|---|
freezing causes loss of activity | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + phenylpyruvate | Agrobacterium tumefaciens | conversion of tryptophan into the plant indolylacetic acid | 3-indole-2-oxopropanoate + L-phenylalanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
67.5fold | Agrobacterium tumefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Agrobacterium tumefaciens |
Storage Stability | Organism |
---|---|
-30°C, crude enzyme, stable for more than 3 months | Agrobacterium tumefaciens |
0-4°C, more than 72 h, partially purified enzyme loses activity | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + 2-oxoglutarate | - |
Agrobacterium tumefaciens | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
L-tryptophan + 2-oxoisopentanoate | i.e. L-Trp + dimethylpyruvate | Agrobacterium tumefaciens | 3-indole-2-oxopropanoate + L-valine | - |
r | |
L-tryptophan + p-hydroxyphenylpyruvate | - |
Agrobacterium tumefaciens | 3-indole-2-oxopropanoate + tyrosine | - |
r | |
L-tryptophan + phenylpyruvate | phenylpyruvate is the most effective amino acceptor, in the reverse reaction phenylalanine is the most effective amino donor | Agrobacterium tumefaciens | 3-indole-2-oxopropanoate + L-phenylalanine | i.e. indolepyruvate + L-Phe | r | |
L-tryptophan + phenylpyruvate | conversion of tryptophan into the plant indolylacetic acid | Agrobacterium tumefaciens | 3-indole-2-oxopropanoate + L-phenylalanine | - |
? | |
additional information | glutamic acid, aspartic acid, alanine, threonine, arginine, glutamine and serine are practically inactive | Agrobacterium tumefaciens | ? | - |
? | |
phenylpyruvate + DL-histidine | low activity | Agrobacterium tumefaciens | phenylalanine + 3-(1H-imidazol-4-yl)-2-oxopropanoate | - |
r | |
phenylpyruvate + DL-isoleucine | - |
Agrobacterium tumefaciens | phenylalanine + 3-methyl-2-oxopentanoate | - |
r | |
phenylpyruvate + DL-leucine | - |
Agrobacterium tumefaciens | phenylalanine + 4-methyl-2-oxopentanoate | - |
r | |
phenylpyruvate + DL-methionine | - |
Agrobacterium tumefaciens | phenylalanine + 4-methylsulfanyl-2-oxobutanoate | - |
r | |
phenylpyruvate + DL-valine | - |
Agrobacterium tumefaciens | phenylalanine + 3-methyl-2-oxobutanoate | - |
r | |
phenylpyruvate + L-tyrosine | - |
Agrobacterium tumefaciens | phenylalanine + 3-(4-hydroxyphenyl)-2-oxopropanoate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
increase in activity from 20°C to 70°C | Agrobacterium tumefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
markedly heat-stable in presence of tryptophan, phenylpyruvate or pyridoxal 5'-phosphate | Agrobacterium tumefaciens |
45 | - |
30 min, with pyridoxal 5'-phosphate, no loss of activity | Agrobacterium tumefaciens |
70 | - |
5 min, without substrate or pyridoxal 5'-phosphate, complete loss of activity | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
- |
Agrobacterium tumefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.6 | 10.6 | pH 8.6: about 70% of activity maximum, pH 10.6: about 80% of activity maximum | Agrobacterium tumefaciens |