BRENDA - Enzyme Database show
show all sequences of 2.6.1.27

Purification, characteriaztion and identification of tryptophan aminotransferase from rat brain

Minatogawa, Y.; Noguchi, T.; Kido, R.; J. Neurochem. 27, 1097-1101 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
L-glutamate
inhibits activity towards tryptophan
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
also synaptosomal cytosol
Rattus norvegicus
5829
-
additional information
no activity in mitochondria
Rattus norvegicus
-
-
synaptosome
cytosol
Rattus norvegicus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
-
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxytryptophan + 2-oxoglutarate
-
639946
Rattus norvegicus
3-(5-hydroxyindole)-2-oxopropanoate + L-glutamate
-
-
-
?
5-hydroxytryptophan + oxaloacetate
-
639946
Rattus norvegicus
3-(5-hydroxyindole)-2-oxopropanoate + L-aspartate
-
-
-
?
L-aspartate + 2-oxoglutarate
-
639946
Rattus norvegicus
oxaloacetate + L-glutamate
-
639946
Rattus norvegicus
?
L-aspartate + oxaloacetate
-
639946
Rattus norvegicus
oxaloacetate + L-aspartate
-
639946
Rattus norvegicus
?
L-phenylalanine + 2-oxoglutarate
-
639946
Rattus norvegicus
L-glutamate + phenylpyruvate
-
-
-
?
L-phenylalanine + oxaloacetate
-
639946
Rattus norvegicus
phenylpyruvate + L-aspartate
-
-
-
?
L-tryptophan + 2-oxoglutarate
-
639946
Rattus norvegicus
L-glutamate + 3-indole-2-oxopropanoate
-
-
-
?
L-tryptophan + oxaloacetate
-
639946
Rattus norvegicus
3-indole-2-oxopropanoate + L-aspartate
-
-
-
?
L-tyrosine + 2-oxoglutarate
-
639946
Rattus norvegicus
L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
-
-
?
L-tyrosine + oxaloacetate
-
639946
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-aspartate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Rattus norvegicus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
-
-
6.2
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-glutamate
inhibits activity towards tryptophan
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
also synaptosomal cytosol
Rattus norvegicus
5829
-
additional information
no activity in mitochondria
Rattus norvegicus
-
-
synaptosome
cytosol
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
-
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxytryptophan + 2-oxoglutarate
-
639946
Rattus norvegicus
3-(5-hydroxyindole)-2-oxopropanoate + L-glutamate
-
-
-
?
5-hydroxytryptophan + oxaloacetate
-
639946
Rattus norvegicus
3-(5-hydroxyindole)-2-oxopropanoate + L-aspartate
-
-
-
?
L-aspartate + 2-oxoglutarate
-
639946
Rattus norvegicus
oxaloacetate + L-glutamate
-
639946
Rattus norvegicus
?
L-aspartate + oxaloacetate
-
639946
Rattus norvegicus
oxaloacetate + L-aspartate
-
639946
Rattus norvegicus
?
L-phenylalanine + 2-oxoglutarate
-
639946
Rattus norvegicus
L-glutamate + phenylpyruvate
-
-
-
?
L-phenylalanine + oxaloacetate
-
639946
Rattus norvegicus
phenylpyruvate + L-aspartate
-
-
-
?
L-tryptophan + 2-oxoglutarate
-
639946
Rattus norvegicus
L-glutamate + 3-indole-2-oxopropanoate
-
-
-
?
L-tryptophan + oxaloacetate
-
639946
Rattus norvegicus
3-indole-2-oxopropanoate + L-aspartate
-
-
-
?
L-tyrosine + 2-oxoglutarate
-
639946
Rattus norvegicus
L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
-
-
?
L-tyrosine + oxaloacetate
-
639946
Rattus norvegicus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-aspartate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Rattus norvegicus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rattus norvegicus
-
-
6.2
Other publictions for EC 2.6.1.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738172
Preuss
Characterization of tryptophan ...
Malassezia furfur, Malassezia furfur CBS 7019
Exp. Dermatol.
22
736-741
2013
-
-
1
-
-
-
2
2
-
-
2
-
-
8
-
-
-
-
-
-
-
1
8
1
1
-
-
-
1
-
-
1
-
-
2
-
-
1
1
-
-
-
2
2
-
2
-
-
2
-
-
-
-
-
-
-
-
1
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
723349
Abu-Zaitoon
A large increase in IAA during ...
Oryza sativa
Physiol. Plant.
146
487-499
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
720687
He
A small-molecule screen identi ...
Arabidopsis thaliana
Plant Cell
23
3944-3960
2011
-
-
1
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
720689
Phillips
Vanishing tassel2 encodes a gr ...
Zea mays
Plant Cell
23
550-566
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
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-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720922
Won
Conversion of tryptophan to in ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
108
18518-18523
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
702493
Kumavath
L-Tryptophan catabolism by Rub ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
Biodegradation
21
825-832
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689054
Zuther
The tryptophan aminotransferas ...
Ustilago maydis
Mol. Microbiol.
68
152-172
2008
-
-
-
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639941
Koshiba
-
L- And D-tryptophan aminotrans ...
Zea mays
J. Plant Res.
106
25-29
1993
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
1
-
-
5
-
2
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
5
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
639942
Frankenberger
-
L-Tryptophan transaminase of a ...
Bacteria
Soil Biol. Biochem.
20
299-304
1988
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639943
Bode
-
Characterization of three tryp ...
Candida maltosa
Prog. Tryptophan Serotonin Res. Proc. -Meet. Int. Study Group Tryptophan Res. ISTRY (Schlossberger, H. G. , ed. ) 4th, Meeting Date1983, de Gruyter
Berlin
769-772
1984
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639944
Stanley
-
Tryptophan aminotransferase ac ...
Rattus norvegicus
Prog. Tryptophan Serotonin Res. , Proc. -Meet. Int. Study Group Tryptophan Res. ISTRY (Schlossberger, H. G. , ed. ) 4th, Meeting Date1983, de Gruyter
Berlin
665-668
1984
-
-
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639945
Lesch
-
Transamination of L- and D-try ...
Rhodotorula toruloides
Biochem. Physiol. Pflanz.
174
546-554
1979
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
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-
-
-
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-
2
-
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-
-
-
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1
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1
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-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639946
Minatogawa
Purification, characteriaztion ...
Rattus norvegicus
J. Neurochem.
27
1097-1101
1976
-
-
-
-
-
-
1
-
3
-
1
-
-
2
-
-
1
-
-
2
-
-
10
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
1
-
2
-
-
10
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
441312
Speedie
Isolation and characterization ...
Streptomyces griseus
J. Biol. Chem.
250
7819-7825
1975
-
-
-
-
-
-
2
-
-
-
-
1
-
3
-
-
1
-
-
-
-
1
6
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
1
6
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
639947
Truelsen
-
Indole-3-pyruvic acid as an in ...
Vigna radiata var. radiata
Physiol. Plant.
26
289-295
1972
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
3
-
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2
-
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-
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1
1
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1
-
3
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639938
George
Brain aromatic aminotransferas ...
Sus scrofa
Biochim. Biophys. Acta
167
555-566
1968
3
-
-
-
-
-
3
6
-
-
-
-
-
2
-
-
1
-
-
3
1
2
11
-
1
-
-
-
2
-
-
2
-
-
-
3
-
-
2
-
-
-
-
3
-
6
-
-
-
-
-
-
-
1
-
3
1
2
11
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
639939
O'Neil
Tryptophan transaminase from C ...
Clostridium sporogenes 175, Clostridium sporogenes
Arch. Biochem. Biophys.
127
361-369
1968
1
-
-
-
-
2
-
2
-
-
1
2
-
3
-
-
1
-
-
-
1
1
8
-
1
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
2
-
-
-
2
-
-
1
2
-
-
-
1
-
-
1
1
8
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-