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Literature summary for 2.6.1.27 extracted from

  • Speedie, M.K.; Hornemann, U.; Floss, H.G.
    Isolation and characterization of tryptophan transaminase and indolepyruvate C-methyltransferase. Enzymes involved in indolmycin biosynthesis in Streptomyces griseus (1975), J. Biol. Chem., 250, 7819-7825.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
3-Methyltryptophan can also act as substrate Streptomyces griseus
p-chloromercuribenzoate no inhibition Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + 2-oxoglutarate Streptomyces griseus initial step of biosynthetic pathway of the antibiotic indolmycin L-glutamate + 3-indole-2-oxopropanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
 strain ATCC 12648
-

Purification (Commentary)

Purification (Comment) Organism
partial, 3fold Streptomyces griseus

Storage Stability

Storage Stability Organism
-20°C, best stored by freezing the crude cell-free extract with 10% glycerol or by freezing the 45-60% ammonium sulfate precipitate in a phosphate buffer solution Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyltryptophan + 2-oxoglutarate can also inhibit the enzyme, stereospecific for positions 2 and 3 Streptomyces griseus 3-(3-methylindole)-2-oxopropanoate + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Streptomyces griseus L-glutamate + phenylpyruvate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Streptomyces griseus L-glutamate + 3-indole-2-oxopropanoate
-
 ?
L-tryptophan + 2-oxoglutarate initial step of biosynthetic pathway of the antibiotic indolmycin Streptomyces griseus L-glutamate + 3-indole-2-oxopropanoate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Streptomyces griseus L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
 ?
additional information no activity with D-tryptophan Streptomyces griseus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 10 12 h, complete loss of activity Streptomyces griseus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Streptomyces griseus