BRENDA - Enzyme Database show
show all sequences of 2.6.1.24

Purification and properties of halogenated tyrosine and thyroid hormone transaminase from rat kidney mitochondria

Nakano, M.; J. Biol. Chem. 242, 73-81 (1967)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3,5-Diiodo-4-hydroxyphenyl-DL-lactic acid
-
Rattus norvegicus
3,5-Diiodo-4-hydroxyphenylacetate
strong inhibition, mixed type
Rattus norvegicus
4-chloromercuribenzoate
-
Rattus norvegicus
4-hydroxyphenyl-DL-lactic acid
weak
Rattus norvegicus
4-hydroxyphenylacetic acid
-
Rattus norvegicus
o-Iodosobenzoate
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
1.3
3,5-diiodo-L-tyrosine
pH 6.2, 37°C
Rattus norvegicus
3.4
3.7
3,5-Dibromo-L-tyrosine
pH 6.5, 37°C
Rattus norvegicus
6.1
-
3,5-dichloro-L-tyrosine
pH 6.-L-7, 37°C
Rattus norvegicus
6.5
-
3-iodo-L-tyrosine
pH 7.4, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,5-diiodo-L-tyrosine + 2-oxoglutarate
Rattus norvegicus
-
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
30fold
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.53
-
partially purified enzyme
Rattus norvegicus
Storage Stability
Storage Stability
Organism
0°C, 50 mM sodium phosphate buffer, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.15 M NaCl, approximately 4 mg protein/ml, 40% loss of activity after 1 week
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,5-dibromo-L-tyrosine + 2-oxoglutarate
at 127% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3,5-dibromo-4-hydroxyphenylpyruvate + L-glutamate
-
-
-
?
3,5-dichloro-L-tyrosine + 2-oxoglutarate
at 110% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3,5-dichloro-4-hydroxyphenylpyruvate + L-glutamate
-
-
-
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
-
639936
Rattus norvegicus
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
-
639936
Rattus norvegicus
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
specific for 2-oxoglutarate as amino acceptor
639936
Rattus norvegicus
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
-
639936
Rattus norvegicus
?
3,5-diiodo-L-tyrosine + oxaloacetate
30% substitution of 2-oxoglutarate by oxaloacetate as amino acceptor
639936
Rattus norvegicus
3,5-diiodo-4-hydroxyphenylpyruvate + L-aspartate
-
639936
Rattus norvegicus
?
3-(4-fluorophenyl)alanine + 2-oxoglutarate
i.e. p-fluoro-DL-phenylalanine, at 3.1% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
(4-fluorophenyl)pyruvate + L-glutamate
-
-
-
?
3-iodo-L-tyrosine + 2-oxoglutarate
at 76% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3-iodo-4-hydroxyphenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + 2-oxoglutarate
at 3.9% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
phenylpyruvate + L-glutamate
-
-
-
?
L-tryptophan + 2-oxoglutarate
at 3.6% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3-indole-2-oxopropanoate + L-glutamate
-
-
-
?
L-tyrosine + 2-oxoglutarate
at 3.9% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
(4-hydroxyphenyl)pyruvate + L-glutamate
-
-
-
?
additional information
substrate specificity
639936
Rattus norvegicus
?
-
-
-
-
additional information
no activity with: D-diiodotyrosine, tyrosine analogues with nitro, amino or hydroxy substituents in position 3 or 3 and 5
639936
Rattus norvegicus
?
-
-
-
-
additional information
no activity with pyruvate as amino acceptor
639936
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
with substrate diiodo-L-tyrosine
Rattus norvegicus
6.5
-
with substrate dibromo-L-tyrosine, dichloro-L-tyrosine
Rattus norvegicus
7
7.5
with substrate monoiodo-L-tyrosine
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,5-Diiodo-4-hydroxyphenyl-DL-lactic acid
-
Rattus norvegicus
3,5-Diiodo-4-hydroxyphenylacetate
strong inhibition, mixed type
Rattus norvegicus
4-chloromercuribenzoate
-
Rattus norvegicus
4-hydroxyphenyl-DL-lactic acid
weak
Rattus norvegicus
4-hydroxyphenylacetic acid
-
Rattus norvegicus
o-Iodosobenzoate
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
1.3
3,5-diiodo-L-tyrosine
pH 6.2, 37°C
Rattus norvegicus
3.4
3.7
3,5-Dibromo-L-tyrosine
pH 6.5, 37°C
Rattus norvegicus
6.1
-
3,5-dichloro-L-tyrosine
pH 6.-L-7, 37°C
Rattus norvegicus
6.5
-
3-iodo-L-tyrosine
pH 7.4, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,5-diiodo-L-tyrosine + 2-oxoglutarate
Rattus norvegicus
-
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
30fold
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.53
-
partially purified enzyme
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 50 mM sodium phosphate buffer, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.15 M NaCl, approximately 4 mg protein/ml, 40% loss of activity after 1 week
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,5-dibromo-L-tyrosine + 2-oxoglutarate
at 127% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3,5-dibromo-4-hydroxyphenylpyruvate + L-glutamate
-
-
-
?
3,5-dichloro-L-tyrosine + 2-oxoglutarate
at 110% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3,5-dichloro-4-hydroxyphenylpyruvate + L-glutamate
-
-
-
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
-
639936
Rattus norvegicus
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
-
639936
Rattus norvegicus
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
specific for 2-oxoglutarate as amino acceptor
639936
Rattus norvegicus
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
-
639936
Rattus norvegicus
?
3,5-diiodo-L-tyrosine + oxaloacetate
30% substitution of 2-oxoglutarate by oxaloacetate as amino acceptor
639936
Rattus norvegicus
3,5-diiodo-4-hydroxyphenylpyruvate + L-aspartate
-
639936
Rattus norvegicus
?
3-(4-fluorophenyl)alanine + 2-oxoglutarate
i.e. p-fluoro-DL-phenylalanine, at 3.1% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
(4-fluorophenyl)pyruvate + L-glutamate
-
-
-
?
3-iodo-L-tyrosine + 2-oxoglutarate
at 76% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3-iodo-4-hydroxyphenylpyruvate + L-glutamate
-
-
-
?
L-phenylalanine + 2-oxoglutarate
at 3.9% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
phenylpyruvate + L-glutamate
-
-
-
?
L-tryptophan + 2-oxoglutarate
at 3.6% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
3-indole-2-oxopropanoate + L-glutamate
-
-
-
?
L-tyrosine + 2-oxoglutarate
at 3.9% the rate of 3,5-diiodo-L-tyrosine
639936
Rattus norvegicus
(4-hydroxyphenyl)pyruvate + L-glutamate
-
-
-
?
additional information
substrate specificity
639936
Rattus norvegicus
?
-
-
-
-
additional information
no activity with: D-diiodotyrosine, tyrosine analogues with nitro, amino or hydroxy substituents in position 3 or 3 and 5
639936
Rattus norvegicus
?
-
-
-
-
additional information
no activity with pyruvate as amino acceptor
639936
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
with substrate diiodo-L-tyrosine
Rattus norvegicus
6.5
-
with substrate dibromo-L-tyrosine, dichloro-L-tyrosine
Rattus norvegicus
7
7.5
with substrate monoiodo-L-tyrosine
Rattus norvegicus
Other publictions for EC 2.6.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639936
Nakano
Purification and properties of ...
Rattus norvegicus
J. Biol. Chem.
242
73-81
1967
-
-
-
-
-
-
6
4
1
-
1
1
-
1
-
-
1
-
-
1
1
1
13
-
1
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
4
1
-
1
1
-
-
-
1
-
1
1
1
13
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-