BRENDA - Enzyme Database show
show all sequences of 2.6.1.23

Metabolism of gamma-hydroxyglutamic acid. I. Conversion to alpha-hydroxy-gamma-ketoglutarate by purified glutamic-aspartic transaminase of rat liver

Goldstone, A.; Adams, E.; J. Biol. Chem. 237, 3476-3485 (1962)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
hydroxylamine
70% inhibition at 1 mM
Rattus norvegicus
L-glutarate
63% inhibition at 0.5 mM
Rattus norvegicus
Maleate
65% inhibition at 5 mM
Rattus norvegicus
N-ethylmaleimide
50% inhibition at 5 mM
Rattus norvegicus
p-chloromercuribenzoate
77% inhibition at 0.5 mM
Rattus norvegicus
phenylhydrazine
84% inhibition at 1 mM
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
2-oxoglutarate
pH 8.1, 37°C
Rattus norvegicus
33
-
L-glutamate
pH 8.1, 37°C
Rattus norvegicus
240
-
erythro-L-hydroxyglutamic acid
pH 8.1, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxy-L-glutamate + oxo acid
Rattus norvegicus
metabolism of 4-hydroxy-L-glutamate in rat liver
4-hydroxy-2-oxoglutarate + L-glutamate
-
Rattus norvegicus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.49
-
partially purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxy-L-glutamate + 2-oxoglutarate
enzyme utilises erythro-L-4-hydroxyglutamate and threo-L-4-hydroxyglutamate to the same extent, but no D-isomers
639934
Rattus norvegicus
4-hydroxy-2-oxoglutarate + L-glutamate
-
639934
Rattus norvegicus
r
4-hydroxy-L-glutamate + oxaloacetate
-
639934
Rattus norvegicus
4-hydroxy-2-oxoglutarate + L-aspartate
-
-
-
r
4-hydroxy-L-glutamate + oxo acid
metabolism of 4-hydroxy-L-glutamate in rat liver
639934
Rattus norvegicus
4-hydroxy-2-oxoglutarate + L-glutamate
-
639934
Rattus norvegicus
r
additional information
substrate stereospecificity
639934
Rattus norvegicus
?
-
-
-
-
additional information
pyruvate is a poor substrate
639934
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
with erythro-L-hydroxyglutamic acid
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.7
9.5
pH 6.7: about 45% of activity maximum, pH 9.5: about 55% of activity maximum
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor requirement
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor requirement
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydroxylamine
70% inhibition at 1 mM
Rattus norvegicus
L-glutarate
63% inhibition at 0.5 mM
Rattus norvegicus
Maleate
65% inhibition at 5 mM
Rattus norvegicus
N-ethylmaleimide
50% inhibition at 5 mM
Rattus norvegicus
p-chloromercuribenzoate
77% inhibition at 0.5 mM
Rattus norvegicus
phenylhydrazine
84% inhibition at 1 mM
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
2-oxoglutarate
pH 8.1, 37°C
Rattus norvegicus
33
-
L-glutamate
pH 8.1, 37°C
Rattus norvegicus
240
-
erythro-L-hydroxyglutamic acid
pH 8.1, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxy-L-glutamate + oxo acid
Rattus norvegicus
metabolism of 4-hydroxy-L-glutamate in rat liver
4-hydroxy-2-oxoglutarate + L-glutamate
-
Rattus norvegicus
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.49
-
partially purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxy-L-glutamate + 2-oxoglutarate
enzyme utilises erythro-L-4-hydroxyglutamate and threo-L-4-hydroxyglutamate to the same extent, but no D-isomers
639934
Rattus norvegicus
4-hydroxy-2-oxoglutarate + L-glutamate
-
639934
Rattus norvegicus
r
4-hydroxy-L-glutamate + oxaloacetate
-
639934
Rattus norvegicus
4-hydroxy-2-oxoglutarate + L-aspartate
-
-
-
r
4-hydroxy-L-glutamate + oxo acid
metabolism of 4-hydroxy-L-glutamate in rat liver
639934
Rattus norvegicus
4-hydroxy-2-oxoglutarate + L-glutamate
-
639934
Rattus norvegicus
r
additional information
substrate stereospecificity
639934
Rattus norvegicus
?
-
-
-
-
additional information
pyruvate is a poor substrate
639934
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
with erythro-L-hydroxyglutamic acid
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.7
9.5
pH 6.7: about 45% of activity maximum, pH 9.5: about 55% of activity maximum
Rattus norvegicus
Other publictions for EC 2.6.1.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639935
Kuratomi
The metabolism of gamma hydrox ...
Rattus norvegicus
Biochim. Biophys. Acta
78
629-636
1963
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639934
Goldstone
Metabolism of gamma-hydroxyglu ...
Rattus norvegicus
J. Biol. Chem.
237
3476-3485
1962
-
-
-
-
-
-
6
3
1
-
-
1
-
1
-
-
1
-
-
1
1
-
5
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
3
1
-
-
1
-
-
-
1
-
1
1
-
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-