Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
expressed in Escherichia coli as a His-tagged fusion protein | Zea mays |
expressed in Escherichia coli as a His-tagged fusion protein | Rhodopseudomonas palustris |
expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas putida |
expressed in Escherichia coli as a His-tagged fusion protein | Oryza sativa |
expressed in Escherichia coli as a His-tagged fusion protein | Halalkalibacterium halodurans |
expressed in Escherichia coli as a His-tagged fusion protein | Hordeum vulgare |
Crystallization (Comment) | Organism |
---|---|
HvAlaAT is crystallized in complex with pyridoxal 5'-phosphate and L-cycloserine. Structure of this complex is solved at 2.7 A resolution | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(aminooxy)acetate | - |
Hordeum vulgare | |
(aminooxy)acetate | - |
Saccharomyces cerevisiae | |
cycloserine | - |
Hordeum vulgare | |
cycloserine | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
2-oxoglutarate | 25°C, pH 8.0 | Pseudomonas putida | |
0.08 | - |
pyruvate | 25°C, pH 8.0 | Rhodopseudomonas palustris | |
0.08 | - |
pyruvate | 25°C, pH 8.0 | Halalkalibacterium halodurans | |
0.11 | - |
pyruvate | 25°C, pH 8.0 | Oryza sativa | |
0.2 | - |
pyruvate | 25°C, pH 8.0 | Pseudomonas putida | |
0.2 | - |
pyruvate | 25°C, pH 8.0 | Hordeum vulgare | |
0.2 | - |
2-oxoglutarate | 25°C, pH 8.0 | Saccharomyces cerevisiae | |
0.2 | - |
2-oxoglutarate | 25°C, pH 8.0 | Zea mays | |
0.2 | - |
2-oxoglutarate | 25°C, pH 8.0 | Rhodopseudomonas palustris | |
0.2 | - |
L-glutamate | 25°C, pH 8.0 | Rhodopseudomonas palustris | |
0.21 | - |
L-alanine | 25°C, pH 8.0 | Pseudomonas putida | |
0.24 | - |
L-glutamate | 25°C, pH 8.0 | Saccharomyces cerevisiae | |
0.25 | - |
2-oxoglutarate | 25°C, pH 8.0 | Oryza sativa | |
0.3 | - |
2-oxoglutarate | 25°C, pH 8.0 | Hordeum vulgare | |
0.32 | - |
2-oxoglutarate | 25°C, pH 8.0 | Halalkalibacterium halodurans | |
0.37 | - |
2-oxoglutarate | 25°C, pH 8.0 | Zea mays | |
0.4 | - |
pyruvate | 25°C, pH 8.0 | Saccharomyces cerevisiae | |
0.4 | - |
L-alanine | 25°C, pH 8.0 | Oryza sativa | |
0.42 | - |
pyruvate | 25°C, pH 8.0 | Zea mays | |
0.44 | - |
L-glutamate | 25°C, pH 8.0 | Halalkalibacterium halodurans | |
0.54 | - |
pyruvate | 25°C, pH 8.0 | Zea mays | |
0.55 | - |
L-glutamate | 25°C, pH 8.0 | Zea mays | |
0.56 | - |
L-glutamate | 25°C, pH 8.0 | Zea mays | |
0.57 | - |
L-glutamate | 25°C, pH 8.0 | Pseudomonas putida | |
0.62 | - |
L-alanine | 25°C, pH 8.0 | Zea mays | |
0.68 | - |
L-glutamate | 25°C, pH 8.0 | Oryza sativa | |
0.8 | - |
L-glutamate | 25°C, pH 8.0 | Hordeum vulgare | |
1.5 | 2 | L-alanine | 25°C, pH 8.0 | Rhodopseudomonas palustris | |
3.06 | - |
L-alanine | 25°C, pH 8.0 | Zea mays | |
3.6 | - |
L-alanine | 25°C, pH 8.0 | Saccharomyces cerevisiae | |
3.8 | - |
L-alanine | 25°C, pH 8.0 | Hordeum vulgare | |
5.15 | - |
L-alanine | 25°C, pH 8.0 | Halalkalibacterium halodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
- |
- |
Hordeum vulgare | P52894 | - |
- |
Oryza sativa | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Rhodopseudomonas palustris | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Zea mays | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | - |
Saccharomyces cerevisiae | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxoglutarate | - |
Zea mays | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxoglutarate | - |
Rhodopseudomonas palustris | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxoglutarate | - |
Pseudomonas putida | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxoglutarate | - |
Oryza sativa | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxoglutarate | - |
Halalkalibacterium halodurans | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxoglutarate | - |
Hordeum vulgare | pyruvate + L-glutamate | - |
r | |
additional information | enzyme is also able to utilize aspartate and oxaloacetate with 10% efficiency as compared to the native substrates | Hordeum vulgare | ? | - |
? | |
pyruvate + L-glutamate | - |
Hordeum vulgare | L-alanine + 2-oxoglutarate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Bh D-AlaAT | - |
Halalkalibacterium halodurans |
HvAlaAT | - |
Hordeum vulgare |
OsAlaAT | - |
Oryza sativa |
Pp beta-AlaAT | - |
Pseudomonas putida |
Rp D-AlaAT | - |
Rhodopseudomonas palustris |
ScAlaAT | - |
Saccharomyces cerevisiae |
ZmAlaAT1 | - |
Zea mays |
ZmAlaAT2 | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Pseudomonas putida | |
0.08 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Pseudomonas putida | |
0.17 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Pseudomonas putida | |
0.17 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Pseudomonas putida | |
0.33 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Zea mays | |
0.33 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Zea mays | |
0.38 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Rhodopseudomonas palustris | |
0.38 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Rhodopseudomonas palustris | |
0.67 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Zea mays | |
0.67 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Zea mays | |
1.25 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Halalkalibacterium halodurans | |
1.25 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Halalkalibacterium halodurans | |
2.8 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Halalkalibacterium halodurans | |
2.8 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Halalkalibacterium halodurans | |
3.06 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Rhodopseudomonas palustris | |
3.06 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Rhodopseudomonas palustris | |
4.98 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Oryza sativa | |
4.98 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Oryza sativa | |
5.49 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Zea mays | |
5.49 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Zea mays | |
6.09 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Saccharomyces cerevisiae | |
6.09 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Saccharomyces cerevisiae | |
6.56 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Zea mays | |
6.56 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Zea mays | |
6.86 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Oryza sativa | |
6.86 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Oryza sativa | |
8.64 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Saccharomyces cerevisiae | |
8.64 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Saccharomyces cerevisiae | |
12.11 | - |
L-alanine | cofactor: 2-oxoglutarate, 25°C, pH 8.0 | Hordeum vulgare | |
12.11 | - |
2-oxoglutarate | cofactor: L-alanine, 25°C, pH 8.0 | Hordeum vulgare | |
25.62 | - |
pyruvate | cofactor: L-glutamate, 25°C, pH 8.0 | Hordeum vulgare | |
25.62 | - |
L-glutamate | cofactor: pyruvate, 25°C, pH 8.0 | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae | |
pyridoxal 5'-phosphate | - |
Zea mays | |
pyridoxal 5'-phosphate | - |
Rhodopseudomonas palustris | |
pyridoxal 5'-phosphate | - |
Pseudomonas putida | |
pyridoxal 5'-phosphate | - |
Oryza sativa | |
pyridoxal 5'-phosphate | - |
Halalkalibacterium halodurans | |
pyridoxal 5'-phosphate | - |
Hordeum vulgare |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0069 | - |
25°C, pH 8.0 | Hordeum vulgare | (aminooxy)acetate | |
0.119 | - |
25°C, pH 8.0 | Hordeum vulgare | cycloserine |