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Literature summary for 2.6.1.2 extracted from

  • Tamaki, N.; Aoyama, H.; Kubo, K.; Ikeda, T.; Hama, T.
    Purification and properties of beta-alanine aminotransferase from rabbit liver (1982), J. Biochem., 92, 1009-1017.
    View publication on PubMed
Search for more literature for 2.6.1.2

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate substrate inhibition at neutral pH, not seen at pH 8.8 Oryctolagus cuniculus
6-Azauracil 1.5 mM, 50% inhibition Oryctolagus cuniculus
additional information not inhibited by 6-azauridine or 6-azauridine 5'-phosphate Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 4-aminobutanoate pH 8.8, 37°C, cosubstrate 2-oxoglutarate Oryctolagus cuniculus
0.54
-
 2-oxoglutarate pH 8.8, 37°C Oryctolagus cuniculus
1.4
-
 2-oxoglutarate pH 8.8, 37°C, cosubstrate beta-alanine Oryctolagus cuniculus
3.9
-
 beta-Alanine pH 8.8, 37°C, cosubstrate 2-oxoglutarate Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Oryctolagus cuniculus
95000
-
 gel filtration Oryctolagus cuniculus
118000
-
 sucrose density gradient centrifugation Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 2-oxoglutarate Oryctolagus cuniculus important function in beta-alanine metabolism pyruvate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment, ammonium sulfate, DEAE-Sepharose, hydroxylapatite, Sephacryl S-200 Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
L-alanine + 2-oxoglutarate = pyruvate + L-glutamate ping pong bi bi mechanism Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.58
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-25°C, 10 mM potassium phosphate, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal 5'-phosphate, a few days, 50% loss of activity Oryctolagus cuniculus
4°C, 80% saturated solution of ammonium sulfate, 10 days, 40% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + 5-aminopentanoate 121% of the activity with beta-alanine Oryctolagus cuniculus glutamate + 5-oxopentanoate
-
 ?
2-oxoglutarate + 6-aminohexanoate 8% of the activity with beta-alanine Oryctolagus cuniculus glutamate + 6-oxohexanoate
-
 ?
2-oxoglutarate + DL-3-aminoisobutanoate 51% of the activity with beta-alanine Oryctolagus cuniculus glutamate + 3-oxoisobutanoate
-
 ?
4-aminobutanoate + 2-oxoglutarate at 131% of the activity with beta-alanine Oryctolagus cuniculus 4-oxobutanoate + glutamate
-
 ?
beta-alanine + glyoxylic acid 7% of the activity with 2-oxoglutarate Oryctolagus cuniculus malonic semialdehyde + glycine
-
 ?
beta-alanine + pyruvate 1% of the activity with 2-oxoglutarate Oryctolagus cuniculus malonic semialdehyde + alanine
-
 ?
L-alanine + 2-oxoglutarate
-
 Oryctolagus cuniculus pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate important function in beta-alanine metabolism Oryctolagus cuniculus pyruvate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 mol of pyridoxal 5'-phosphate per mol of dimer Oryctolagus cuniculus