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Literature summary for 2.6.1.2 extracted from
- Partial purification and initial studies of the tomato L-alanine:2-oxoglutarate aminotransferase (1974), Biochim. Biophys. Acta, 350, 392-399.
General Stability
| General Stability | Organism |
|---|---|
| during the purification procedure the enzyme needs protective substances, i.e. glycerol, 2-mercaptoethanol and alanine | Solanum lycopersicum |
| uneffected by repeated freezing and thawing | Solanum lycopersicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
pyruvate | pH 7.25, 37°C | Solanum lycopersicum |  |
| 0.28 | - |
2-oxoglutarate | pH 7.25, 37°C | Solanum lycopersicum | |
| 2.3 | - |
glutamate | pH 7.25, 37°C | Solanum lycopersicum | |
| 2.8 | - |
alanine | pH 7.25, 37°C | Solanum lycopersicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Solanum lycopersicum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
tomato, 2 alanine aminotransferases, soluble and Triton X-100 extractable | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephadex, Sephadex G-200, partial purification | Solanum lycopersicum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-alanine + 2-oxoglutarate = pyruvate + L-glutamate | ping pong bi bi mechanism | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Solanum lycopersicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 120 | - |
- |
Solanum lycopersicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + 2-oxoglutarate | rate of the forward reaction is 4 times higher than the rate of the reverse reaction, no activity with aspartate, glycine, threonine, leucine, methionine, arginine, phenylalanine, tyrosine, tryptophan, glyoxylate and 2-oxo-3-methylvalerate | Solanum lycopersicum | pyruvate + L-glutamate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Solanum lycopersicum |
| 37 | - |
assay at | Solanum lycopersicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
quickly inactivated, 100 mM alanine gives slight protection | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.5 | - |
Solanum lycopersicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | rapid decrease of activity below pH 7 and above pH 7.5 | Solanum lycopersicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
strong loss of activity below | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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