Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.19 extracted from

  • Nonaka, S.; Someya, T.; Zhou, S.; Takayama, M.; Nakamura, K.; Ezura, H.
    An Agrobacterium tumefaciens strain with gamma-aminobutyric acid transaminase activity shows an enhanced genetic Transformation Ability in Plants (2017), Sci. Rep., 7, 42649 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.19

Application

Application Comment Organism
molecular biology Agrobacterium tumefaciens is used to mediate inter-kingdom DNA transfer in plant genetic engineering. Gamma-aminobutyric acid (GABA) is a negative factor in the Agrobacterium-plant interaction, because it inhibits the DNA transfer. Generation of an Agrobacterium tumefaciens strain expressing the Escherichia coli gene gabT, which introduces GABA transaminase activity and the ability to degrade GABA, is achieved to circumvent the inhibitory effect of GABA Escherichia coli
molecular biology Agrobacterium tumefaciens is used to mediate inter-kingdom DNA transfer in plant genetic engineering. Gamma-aminobutyric acid (GABA) is a negative factor in the Agrobacterium-plant interaction, because it inhibits the DNA transfer. Generation of an Agrobacterium tumefaciens strain expressing the Escherichia coli gene gabT, which introduces GABA transaminase activity and the ability to degrade GABA, is achieved to circumvent the inhibitory effect of GABA Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
gene gabT, recombinant expression in Agrobacterium tumefaciens strain C58 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information Agrobacterium tumefaciens is used to mediate inter-kingdom DNA transfer in plant genetic engineering. Gamma-aminobutyric acid (GABA) is a negative factor in the Agrobacterium-plant interaction, because it inhibits the DNA transfer. Generation of an Agrobacterium tumefaciens strain expressing the Escherichia coli gene gabT, which introduces GABA transaminase activity and the ability to degrade GABA, is achieved to circumvent the inhibitory effect of GABA. GabT activity enhances the T-DNA transfer ability of Agrobacterium tumefaciens Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate Escherichia coli
-
 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate Agrobacterium tumefaciens
-
 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate Agrobacterium tumefaciens GV2260
-
 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate Agrobacterium tumefaciens C58
-
 succinate semialdehyde + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens A0A5B8KQ12 i.e. Agrobacterium tumefaciens
-
Agrobacterium tumefaciens Q8UBL5 i.e. Agrobacterium tumefaciens
-
Agrobacterium tumefaciens C58 Q8UBL5 i.e. Agrobacterium tumefaciens
-
Agrobacterium tumefaciens GV2260 Q8UBL5 i.e. Agrobacterium tumefaciens
-
Escherichia coli P22256
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanoate + 2-oxoglutarate
-
 Escherichia coli succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate
-
 Agrobacterium tumefaciens succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate native enzyme Atu3300 shows very low or no GABA transaminase activity Agrobacterium tumefaciens succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate
-
 Agrobacterium tumefaciens GV2260 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate native enzyme Atu3300 shows very low or no GABA transaminase activity Agrobacterium tumefaciens GV2260 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate
-
 Agrobacterium tumefaciens C58 succinate semialdehyde + L-glutamate
-
 ?
4-aminobutanoate + 2-oxoglutarate native enzyme Atu3300 shows very low or no GABA transaminase activity Agrobacterium tumefaciens C58 succinate semialdehyde + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
Atu3000
-
 Agrobacterium tumefaciens
CG010_026395
-
 Agrobacterium tumefaciens
GABA transaminase
-
 Escherichia coli
GABA transaminase
-
 Agrobacterium tumefaciens
gamma-aminobutyric acid transaminase
-
 Escherichia coli
gamma-aminobutyric acid transaminase
-
 Agrobacterium tumefaciens
gatT
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli
pyridoxal 5'-phosphate
-
 Agrobacterium tumefaciens

General Information

General Information Comment Organism
evolution enzyme Atu3300 is predicted by Pfam to include an Aminotrans_3 (aminotransferase class-III) domain, which is characteristic of aminotransferases Agrobacterium tumefaciens
physiological function GabT activity of the recombinant Escherichia coli enzyme expressed in Agrobacterium tumefaciens enhances the T-DNA transfer ability of Agrobacterium tumefaciens Escherichia coli
physiological function the Agrobacterium tumefaciens ability to mediate inter-kingdom DNA transfer in plant genetic engineering is inhibited by gamm-aminobutyric acid (GABA) Agrobacterium tumefaciens