Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
synthesis | simultaneous expression of gamma-aminobutyric acid aminotransferase gabT and succinic semialdehyde dehydrogenase gabD genes in Escherichia coli. The mixture of both enzymes, called GABase, is the key enzyme for the enzymatic analysis of GABA | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens KCCM 12537 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + pyruvate | - |
Pseudomonas fluorescens | 4-oxobutanoate + L-alanine | - |
? | |
4-aminobutanoate + pyruvate | - |
Pseudomonas fluorescens KCCM 12537 | 4-oxobutanoate + L-alanine | - |
? | |
5-aminovaleric acid + pyruvate | - |
Pseudomonas fluorescens | 5-oxopentanoate + L-alanine | 47.7% of the activity with 4-aminobutanoate | ? | |
5-aminovaleric acid + pyruvate | - |
Pseudomonas fluorescens KCCM 12537 | 5-oxopentanoate + L-alanine | 47.7% of the activity with 4-aminobutanoate | ? | |
additional information | GABase activity against beta-alanine, epsilon-amino-n-caproic acid, L-ornithine, L-lysine, and L-aspartic acid is between 0.3 to 2.3% of the activity against 4-aminobutanoate | Pseudomonas fluorescens | ? | - |
? | |
additional information | GABase activity against beta-alanine, epsilon-amino-n-caproic acid, L-ornithine, L-lysine, and L-aspartic acid is between 0.3 to 2.3% of the activity against 4-aminobutanoate | Pseudomonas fluorescens KCCM 12537 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | so-called GABase, mixture of gamma-aminobutyric acid aminotransferase gabT and succinic semialdehyde dehydrogenase gabD, pH 8.6, 30 min, more than 80% of initial activity | Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | so-called GABase, mixture of gamma-aminobutyric acid aminotransferase gabT and succinic semialdehyde dehydrogenase gabD, more than 75% of initial activity for 24 h at 4°C | Pseudomonas fluorescens |