Cloned (Comment) | Organism |
---|---|
co-expression of the truncated GABA-T cDNA with the GroES/ELmolecular chaperone complex in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells, and GABA-T is expressed in Nicotiana tabacum BY-2 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-Alanine | competitive inhibitor of pyruvate-dependent GABA-T activity | Arabidopsis thaliana | |
glycine | competitive inhibitor of pyruvate-dependent GABA-T activity | Arabidopsis thaliana | |
ornithine | competitive inhibitor of pyruvate-dependent GABA-T activity | Arabidopsis thaliana | |
vigabatrin | competitive inhibitor of pyruvate-dependent GABA-T activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
4-Oxobutanoate | with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
0.11 | - |
glyoxylate | in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
0.14 | - |
pyruvate | in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
0.18 | - |
4-aminobutanoate | with glyoxylate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
0.34 | - |
4-aminobutanoate | with pyruvate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
2.4 | - |
L-alanine | with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94CE5 | subunit | - |
Purification (Comment) | Organism |
---|---|
ProBond nickel resin column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + glyoxylate | - |
Arabidopsis thaliana | 4-oxobutanoate + glycine | - |
? | |
4-aminobutanoate + glyoxylate | - |
Arabidopsis thaliana Wassilewskija | 4-oxobutanoate + glycine | - |
? | |
4-aminobutanoate + glyoxylate | - |
Arabidopsis thaliana Col-0 | 4-oxobutanoate + glycine | - |
? | |
4-aminobutanoate + pyruvate | - |
Arabidopsis thaliana | 4-oxobutanoate + L-alanine | - |
? | |
4-aminobutanoate + pyruvate | - |
Arabidopsis thaliana Wassilewskija | 4-oxobutanoate + L-alanine | - |
? | |
4-aminobutanoate + pyruvate | - |
Arabidopsis thaliana Col-0 | 4-oxobutanoate + L-alanine | - |
? | |
additional information | cannot utilize 2-oxoglutarate as amino acceptor | Arabidopsis thaliana | ? | - |
? | |
additional information | cannot utilize 2-oxoglutarate as amino acceptor | Arabidopsis thaliana Wassilewskija | ? | - |
? | |
additional information | cannot utilize 2-oxoglutarate as amino acceptor | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GABA-T | - |
Arabidopsis thaliana |
gamma-aminobutyrate transaminase | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.9 | - |
4-aminobutanoate | with glyoxylate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
9.7 | - |
glyoxylate | in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
10.6 | - |
pyruvate | in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
10.6 | - |
4-aminobutanoate | with pyruvate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
10.8 | - |
4-Oxobutanoate | with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
15.4 | - |
L-alanine | with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
for pyruvate-dependent GABA-T activity | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
ornithine | - |
Arabidopsis thaliana | |
0.55 | - |
beta-Alanine | - |
Arabidopsis thaliana | |
0.62 | - |
vigabatrin | - |
Arabidopsis thaliana | |
3.7 | - |
glycine | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression is highest in roots and increases as a function of leaf development | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
L-alanine | with 4-oxobutanoate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
31 | - |
4-aminobutanoate | with pyruvate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
39 | - |
4-aminobutanoate | with glyoxylate as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
76 | - |
pyruvate | in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
88 | - |
glyoxylate | in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana | |
770 | - |
4-Oxobutanoate | with L-alanine as cosubstrate, in 50 mM TABS (pH 9), 1.5 mM dithiothreitol, 0.625 mM EDTA, 0.1 mM pyridoxal 5'-phosphate, 10% (v/v) glycerol, at 30°C | Arabidopsis thaliana |