Any feedback?
Literature summary for 2.6.1.19 extracted from
- Soluble gamma-aminobutyric-glutamic transaminase from Pseudomonas fluorescens (1959), J. Biol. Chem., 234, 932-936.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | - |
Pseudomonas fluorescens |  |
| KCN | - |
Pseudomonas fluorescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pseudomonas fluorescens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + 2-oxoglutarate | Pseudomonas fluorescens | inducible enzyme | 4-oxobutanoate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.4 | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + 2-oxoglutarate | - |
Pseudomonas fluorescens | 4-oxobutanoate + L-glutamate | - |
r | |
| 4-aminobutanoate + 2-oxoglutarate | inducible enzyme | Pseudomonas fluorescens | 4-oxobutanoate + L-glutamate | - |
? | |
| additional information | overview | Pseudomonas fluorescens | ? | - |
? | |
| additional information | no amino acceptors are oxaloacetate, pyruvate | Pseudomonas fluorescens | ? | - |
? | |
| additional information | no substrate: lysine | Pseudomonas fluorescens | ? | - |
? | |
| additional information | no amino acceptors are ketomalonic acid, alpha-ketoisovaleric acid | Pseudomonas fluorescens | ? | - |
? | |
| additional information | aspartate, L-2,4-diaminoglutarate, alanine, D-glutamate or malonic semialdehyde cannot replace L-glutamate or succinic semialdehyde in the reverse reaction | Pseudomonas fluorescens | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
| 8.6 | 9 | at 25°C | Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.2 | about half-maximal activity at pH 8 | Pseudomonas fluorescens |
| 8 | 9.2 | about 60% of maximal activity at pH 9.2 | Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | below and above, rapid inactivation | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | no activation by addition of pyridoxal phosphate, but sensitive to carbonyl reagents | Pseudomonas fluorescens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
