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Literature summary for 2.6.1.18 extracted from

  • Bea, H.; Lee, S.; Yun, H.
    Asymmetric synthesis of (R)-3-fluoroalanine from 3-fluoropyruvate using omega-transaminase (2011), Biotechnol. Bioprocess Eng., 16, 291-296.
No PubMed abstract available
Search for more literature for 2.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Vibrio fluvialis

Inhibitors

Inhibitors Comment Organism Structure
acetophenone in the presence of 5 mM acetophenone, the reactivity of the enzyme towards F-pyruvate decreased by 78% Vibrio fluvialis

Organism

Organism UniProt Comment Textmining
Vibrio fluvialis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Vibrio fluvialis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
 specific activity of the purified enzyme is comparable to the purified native enzyme, pH 7, 37°C Vibrio fluvialis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-fluoropyruvate + (1S)-1-phenyl-ethanamine to overcome the product inhibition by acetophenone, a biphasic reaction is used Vibrio fluvialis (R)-3-fluoroalanine + acetophenone
-
 ?

Synonyms

Synonyms Comment Organism
omega-transaminase
-
 Vibrio fluvialis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vibrio fluvialis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Vibrio fluvialis