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Literature summary for 2.6.1.18 extracted from

  • Nakano, Y.; Tokunaga, H.; Kitaoka, S.
    Two omega-amino acid transaminases from Bacillus cereus (1977), J. Biochem., 81, 1375-1381.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cysteine 0.5 mM, 14% inhibition Bacillus cereus
glutathione 0.5 mM, 12% inhibition Bacillus cereus
Monoiodoacetic acid 0.5 mM, 24% inhibition Bacillus cereus
p-chloromercuribenzoate 0.5 mM, 71% inhibition Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
beta-Alanine pH 10.0, 35°C, cosubstrate pyruvate Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
K-22
-
Bacillus cereus K-22
-
K-22
-

Purification (Commentary)

Purification (Comment) Organism
K-22, partial purification Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-alanine + 2-oxobutanoate 2-oxo-n-butyric acid Bacillus cereus malonic semialdehyde + 2-aminobutanoate
-
?
beta-alanine + 2-oxobutanoate at 64% of the activity with pyruvate Bacillus cereus malonic semialdehyde + 2-aminobutanoate
-
?
beta-alanine + 2-oxobutanoate 2-oxo-n-butyric acid Bacillus cereus K-22 malonic semialdehyde + 2-aminobutanoate
-
?
beta-alanine + 2-oxoisopentanoate at 13% of the activity with pyruvate Bacillus cereus malonic semialdehyde + L-valine
-
?
beta-alanine + 2-oxoisopentanoate at 13% of the activity with pyruvate Bacillus cereus K-22 malonic semialdehyde + L-valine
-
?
beta-alanine + 2-oxopentanoate at 27% of the activity with pyruvate Bacillus cereus malonic semialdehyde + L-norvaline
-
?
beta-alanine + 2-oxopentanoate at 27% of the activity with pyruvate Bacillus cereus K-22 malonic semialdehyde + L-norvaline
-
?
beta-alanine + oxaloacetic acid at 115% of the activity with pyruvate Bacillus cereus malonic semialdehyde + L-aspartate
-
?
beta-alanine + oxaloacetic acid at 115% of the activity with pyruvate Bacillus cereus K-22 malonic semialdehyde + L-aspartate
-
?
beta-alanine + pyruvate i.e. 3-aminopropanoate + 2-oxopropanoate Bacillus cereus malonic semialdehyde + L-alanine
-
?
beta-alanine + pyruvate i.e. 3-aminopropanoate + 2-oxopropanoate Bacillus cereus K-22 malonic semialdehyde + L-alanine
-
?
pyruvate + 2-aminoethylphosphonic acid at 28% of the activity with beta-alanine Bacillus cereus L-alanine + 2-oxoethylphosphonic acid
-
?
pyruvate + 4-aminobutanoate at 43% of the activity with beta-alanine Bacillus cereus L-alanine + succinic semialdehyde
-
?
pyruvate + 6-aminohexanoate at 9% of the activity with beta-alanine Bacillus cereus L-alanine + 6-oxohexanoate
-
?
pyruvate + propylamine at 37% of the activity with beta-alanine Bacillus cereus L-alanine + propanal
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10.5 stable Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal phosphate protein, 40% stimulation on addition of the cofactor even after the enzyme has previously been incubated with saturating amounts of pyridoxal phosphate Bacillus cereus