BRENDA - Enzyme Database show
show all sequences of 2.6.1.18

Two omega-amino acid transaminases from Bacillus cereus

Nakano, Y.; Tokunaga, H.; Kitaoka, S.; J. Biochem. 81, 1375-1381 (1977)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cysteine
0.5 mM, 14% inhibition
Bacillus cereus
glutathione
0.5 mM, 12% inhibition
Bacillus cereus
Monoiodoacetic acid
0.5 mM, 24% inhibition
Bacillus cereus
p-chloromercuribenzoate
0.5 mM, 71% inhibition
Bacillus cereus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
beta-Alanine
pH 10.0, 35°C, cosubstrate pyruvate
Bacillus cereus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
-
K-22
-
Bacillus cereus K-22
-
K-22
-
Purification (Commentary)
Commentary
Organism
K-22, partial purification
Bacillus cereus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + 2-oxobutanoate
2-oxo-n-butyric acid
636596
Bacillus cereus
malonic semialdehyde + 2-aminobutanoate
-
636596
Bacillus cereus
?
beta-alanine + 2-oxobutanoate
at 64% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + 2-aminobutanoate
-
636596
Bacillus cereus
?
beta-alanine + 2-oxobutanoate
2-oxo-n-butyric acid
636596
Bacillus cereus K-22
malonic semialdehyde + 2-aminobutanoate
-
636596
Bacillus cereus K-22
?
beta-alanine + 2-oxoisopentanoate
at 13% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + L-valine
-
636596
Bacillus cereus
?
beta-alanine + 2-oxoisopentanoate
at 13% of the activity with pyruvate
636596
Bacillus cereus K-22
malonic semialdehyde + L-valine
-
636596
Bacillus cereus K-22
?
beta-alanine + 2-oxopentanoate
at 27% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + L-norvaline
-
636596
Bacillus cereus
?
beta-alanine + 2-oxopentanoate
at 27% of the activity with pyruvate
636596
Bacillus cereus K-22
malonic semialdehyde + L-norvaline
-
636596
Bacillus cereus K-22
?
beta-alanine + oxaloacetic acid
at 115% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + L-aspartate
-
636596
Bacillus cereus
?
beta-alanine + oxaloacetic acid
at 115% of the activity with pyruvate
636596
Bacillus cereus K-22
malonic semialdehyde + L-aspartate
-
636596
Bacillus cereus K-22
?
beta-alanine + pyruvate
i.e. 3-aminopropanoate + 2-oxopropanoate
636596
Bacillus cereus
malonic semialdehyde + L-alanine
-
636596
Bacillus cereus
?
beta-alanine + pyruvate
i.e. 3-aminopropanoate + 2-oxopropanoate
636596
Bacillus cereus K-22
malonic semialdehyde + L-alanine
-
636596
Bacillus cereus K-22
?
pyruvate + 2-aminoethylphosphonic acid
at 28% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + 2-oxoethylphosphonic acid
-
636596
Bacillus cereus
?
pyruvate + 4-aminobutanoate
at 43% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + succinic semialdehyde
-
636596
Bacillus cereus
?
pyruvate + 6-aminohexanoate
at 9% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + 6-oxohexanoate
-
636596
Bacillus cereus
?
pyruvate + propylamine
at 37% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + propanal
-
636596
Bacillus cereus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Bacillus cereus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable up to
Bacillus cereus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Bacillus cereus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
10.5
stable
Bacillus cereus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
a pyridoxal phosphate protein, 40% stimulation on addition of the cofactor even after the enzyme has previously been incubated with saturating amounts of pyridoxal phosphate
Bacillus cereus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
a pyridoxal phosphate protein, 40% stimulation on addition of the cofactor even after the enzyme has previously been incubated with saturating amounts of pyridoxal phosphate
Bacillus cereus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cysteine
0.5 mM, 14% inhibition
Bacillus cereus
glutathione
0.5 mM, 12% inhibition
Bacillus cereus
Monoiodoacetic acid
0.5 mM, 24% inhibition
Bacillus cereus
p-chloromercuribenzoate
0.5 mM, 71% inhibition
Bacillus cereus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
beta-Alanine
pH 10.0, 35°C, cosubstrate pyruvate
Bacillus cereus
Purification (Commentary) (protein specific)
Commentary
Organism
K-22, partial purification
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-alanine + 2-oxobutanoate
2-oxo-n-butyric acid
636596
Bacillus cereus
malonic semialdehyde + 2-aminobutanoate
-
636596
Bacillus cereus
?
beta-alanine + 2-oxobutanoate
at 64% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + 2-aminobutanoate
-
636596
Bacillus cereus
?
beta-alanine + 2-oxobutanoate
2-oxo-n-butyric acid
636596
Bacillus cereus K-22
malonic semialdehyde + 2-aminobutanoate
-
636596
Bacillus cereus K-22
?
beta-alanine + 2-oxoisopentanoate
at 13% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + L-valine
-
636596
Bacillus cereus
?
beta-alanine + 2-oxoisopentanoate
at 13% of the activity with pyruvate
636596
Bacillus cereus K-22
malonic semialdehyde + L-valine
-
636596
Bacillus cereus K-22
?
beta-alanine + 2-oxopentanoate
at 27% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + L-norvaline
-
636596
Bacillus cereus
?
beta-alanine + 2-oxopentanoate
at 27% of the activity with pyruvate
636596
Bacillus cereus K-22
malonic semialdehyde + L-norvaline
-
636596
Bacillus cereus K-22
?
beta-alanine + oxaloacetic acid
at 115% of the activity with pyruvate
636596
Bacillus cereus
malonic semialdehyde + L-aspartate
-
636596
Bacillus cereus
?
beta-alanine + oxaloacetic acid
at 115% of the activity with pyruvate
636596
Bacillus cereus K-22
malonic semialdehyde + L-aspartate
-
636596
Bacillus cereus K-22
?
beta-alanine + pyruvate
i.e. 3-aminopropanoate + 2-oxopropanoate
636596
Bacillus cereus
malonic semialdehyde + L-alanine
-
636596
Bacillus cereus
?
beta-alanine + pyruvate
i.e. 3-aminopropanoate + 2-oxopropanoate
636596
Bacillus cereus K-22
malonic semialdehyde + L-alanine
-
636596
Bacillus cereus K-22
?
pyruvate + 2-aminoethylphosphonic acid
at 28% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + 2-oxoethylphosphonic acid
-
636596
Bacillus cereus
?
pyruvate + 4-aminobutanoate
at 43% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + succinic semialdehyde
-
636596
Bacillus cereus
?
pyruvate + 6-aminohexanoate
at 9% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + 6-oxohexanoate
-
636596
Bacillus cereus
?
pyruvate + propylamine
at 37% of the activity with beta-alanine
636596
Bacillus cereus
L-alanine + propanal
-
636596
Bacillus cereus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Bacillus cereus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable up to
Bacillus cereus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Bacillus cereus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
10.5
stable
Bacillus cereus
Other publictions for EC 2.6.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737507
Wilding
A beta-alanine batabolism path ...
Pseudomonas sp., Pseudomonas sp. AAC
Appl. Environ. Microbiol.
82
3846-3856
2016
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738138
Mathew
Biochemical characterization o ...
Sphaerobacter thermophilus, Sphaerobacter thermophilus DSM 20745
Enzyme Microb. Technol.
87-88
52-60
2016
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
13
-
-
-
1
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-
-
-
-
-
-
-
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-
1
-
-
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-
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1
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1
-
13
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
737842
Fuchs
Synthesis of (R)- or (S)-valin ...
Arabidopsis thaliana, Bacillus megaterium, Fusarium graminearum
Bioorg. Med. Chem.
22
5558-5562
2014
-
3
-
-
-
-
-
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3
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3
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3
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3
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
737864
Han
Metabolically driven equilibri ...
Ochrobactrum anthropi
Biosci. Biotechnol. Biochem.
78
1788-1790
2014
-
1
1
-
-
-
-
-
-
-
-
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-
1
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1
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1
1
-
-
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-
1
-
-
-
-
-
-
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-
-
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-
-
-
-
-
721915
Cardenas-Fernandez
Immobilization of Escherichia ...
Escherichia coli
Biotechnol. Prog.
28
693-698
2012
-
1
1
-
-
-
-
-
-
-
-
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1
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-
-
-
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-
2
-
1
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1
-
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1
-
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1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
722376
Malik
-
omega-Transaminase-catalyzed k ...
Aspergillus terreus, Ochrobactrum anthropi
Green Chem.
14
2137-2140
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
723317
Seo
Deracemization of unnatural am ...
Vibrio fluvialis
Org. Biomol. Chem.
10
2482-2485
2012
-
1
1
-
-
-
1
-
-
-
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-
1
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1
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1
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1
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1
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1
1
1
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1
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1
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1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
721893
Bea
-
Asymmetric synthesis of (R)-3- ...
Vibrio fluvialis
Biotechnol. Bioprocess Eng.
16
291-296
2011
-
-
1
-
-
-
1
-
-
-
-
-
-
1
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1
-
-
-
1
-
1
-
1
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-
-
1
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-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684253
Hoehne
-
A protection strategy substant ...
Achromobacter denitrificans, Vibrio fluvialis
Adv. Synth. Catal.
350
807-812
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
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12
-
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-
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-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671123
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
63
117-119
2007
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
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1
-
-
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1
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1
1
1
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1
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1
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-
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-
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-
684207
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
F63
117-119
2007
-
-
1
1
-
-
-
-
-
-
2
-
-
2
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1
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1
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1
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2
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1
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672713
Ingram
One-pot synthesis of amino-alc ...
Pseudomonas aeruginosa
Biotechnol. Bioeng.
96
559-569
2006
-
-
1
-
-
1
-
-
-
-
1
2
-
3
-
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1
-
-
-
-
-
8
1
-
-
1
-
2
-
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1
-
-
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-
-
1
1
-
-
1
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
8
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
657597
Yun
omega-Amino acid:pyruvate tran ...
Achromobacter denitrificans, Achromobacter denitrificans Y2k-2
Appl. Environ. Microbiol.
70
2529-2534
2004
-
2
1
-
-
-
3
3
-
-
-
2
-
4
-
-
1
-
-
-
1
-
20
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
1
-
-
-
-
3
-
3
-
-
-
2
-
-
-
1
-
-
1
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658326
Yun
Simultaneous synthesis of enan ...
Vibrio fluvialis, Vibrio fluvialis JS17
Biotechnol. Lett.
25
809-814
2003
-
1
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636597
Ito
Influence of dietary protein l ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
47
275-282
2001
-
-
-
-
-
-
-
-
-
-
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3
-
-
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-
-
3
-
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-
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3
-
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-
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-
636598
West
Role of cytosine deaminase and ...
Burkholderia cepacia
Antonie van Leeuwenhoek
77
1-5
2000
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
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4
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1
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15
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1
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16
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