Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxamine 5'-phosphate | shows 20% of pyridoxal 5'-phosphate activation | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 0.1 mM, 70% inhibition | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
beta-Alanine | pH 8.0, 35°C, cosubstrate pyruvate | Pseudomonas fluorescens | |
62 | - |
pyruvate | pH 8.0, 35°C, cosubstrate beta-alanine | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
ATCC 11250 | - |
Purification (Comment) | Organism |
---|---|
heat treatment, alumina C-gamma gel, protamine sulfate, ammonium sulfate | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56.6 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C, several weeks, no loss of activity | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-alanine + 2-oxobutanoate | at 9% of the activity with pyruvate | Pseudomonas fluorescens | malonic semialdehyde + 2-aminobutanoate | - |
? | |
beta-alanine + 2-oxohexanoate | at 6% of the activity with pyruvate | Pseudomonas fluorescens | malonic semialdehyde + L-norleucine | - |
? | |
beta-alanine + 2-oxoisohexanoate | at 4% of the activity with pyruvate | Pseudomonas fluorescens | malonic semialdehyde + L-isoleucine | - |
? | |
beta-alanine + acetoacetate | at 4% of the activity with pyruvate | Pseudomonas fluorescens | malonic semialdehyde + 3-aminobutanoate | - |
? | |
beta-alanine + glyoxylic acid | at 17% of the activity with pyruvate | Pseudomonas fluorescens | malonic semialdehyde + glycine | - |
? | |
beta-alanine + pyruvate | i.e. 3-aminopropanoate + 2-oxopropanoate | Pseudomonas fluorescens | malonic semialdehyde + L-alanine | - |
r | |
pyruvate + 2-aminoisobutanoate | at 4% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + 2-oxo-3-methylpropanoate | - |
? | |
pyruvate + 4-aminobutanoate | at 118% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + succinic semialdehyde | - |
? | |
pyruvate + 5-aminopentanoate | at 17% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + 5-oxopentanoate | - |
? | |
pyruvate + 6-aminohexanoate | at 134% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + 6-oxohexanoate | - |
? | |
pyruvate + DL-3-aminoisobutanoate | at 160% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + 3-oxoisobutanoate | - |
? | |
pyruvate + glycine | at 15% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + glyoxylate | - |
? | |
pyruvate + L-2,4-diaminobutanoate | at 4% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + ? | - |
? | |
pyruvate + L-lysine | at 7% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + ? | - |
? | |
pyruvate + L-ornithine | at 1% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + ? | - |
? | |
pyruvate + taurine | at 8% of the activity with beta-alanine | Pseudomonas fluorescens | L-alanine + 2-oxoethanesulfonic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-alanine-alpha-alanine transaminase | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | approx. 35% of maximal activity at pH 7.0, approx. 50% of maximal activity at pH 11.0 | Pseudomonas fluorescens |