Literature summary for 2.6.1.17 extracted from

Weyand, S.; Kefala, G.; Weiss, M.S.
The three-dimensional structure of N-succinyldiaminopimelate aminotransferase from Mycobacterium tuberculosis (2007), J. Mol. Biol., 367, 825-838.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
diffration to 2.0 A resolution. The orthorhombic crystals contain one functional dimer in the asymmetric unit. The homodimer displays the typical S-shape of class I pyridoxal 5'-phosphate-binding proteins. The two active sites of the dimer both feature an internal aldimine with the co-factor pyridoxal 5'-phosphate covalently bound to the Lys232, although neither substrate nor cofactor has been added during protein production, purification and crystallization. Nine water molecules are conserved in the active site and form an intricate hydrogen-bonding network with the co-factor and the surrounding amino acid residues	Mycobacterium tuberculosis
sitting drop vapour diffusion method, in the presence of 20% (w/v) PEG-3350, Tris-HCl (pH 8.5) and 200 mM MgCl2	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

diffration to 2.0 A resolution. The orthorhombic crystals contain one functional dimer in the asymmetric unit. The homodimer displays the typical S-shape of class I pyridoxal 5'-phosphate-binding proteins. The two active sites of the dimer both feature an internal aldimine with the co-factor pyridoxal 5'-phosphate covalently bound to the Lys232, although neither substrate nor cofactor has been added during protein production, purification and crystallization. Nine water molecules are conserved in the active site and form an intricate hydrogen-bonding network with the co-factor and the surrounding amino acid residues

Mycobacterium tuberculosis

sitting drop vapour diffusion method, in the presence of 20% (w/v) PEG-3350, Tris-HCl (pH 8.5) and 200 mM MgCl2

Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WPZ5	-	-
Mycobacterium tuberculosis H37Rv	P9WPZ5	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity column chromatography and gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-2-amino-6-oxoheptanedioate + L-glutamate	-	Mycobacterium tuberculosis	N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate	-	?
N-succinyl-2-amino-6-oxoheptanedioate + L-glutamate	-	Mycobacterium tuberculosis H37Rv	N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate	-	?

Subunits

Subunits	Comment	Organism
dimer	crystallization data	Mycobacterium tuberculosis
homodimer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
DAP-AT	-	Mycobacterium tuberculosis
DapC	-	Mycobacterium tuberculosis
Rv0858c	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Mycobacterium tuberculosis