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Literature summary for 2.6.1.16 extracted from

  • Ma, W.; Liu, Y.; Wang, Y.; Lv, X.; Li, J.; Du, G.; Liu, L.
    Combinatorial fine-tuning of GNA1 and GlmS expression by 5-terminus fusion engineering leads to overproduction of N-acetylglucosamine in Bacillus subtilis (2019), Biotechnol. J., 14, e1800264 .
    View publication on PubMed
Search for more literature for 2.6.1.16

Application

Application Comment Organism
synthesis the enzyme is useful in bioproduction of N-acetylglucosamine (GlcNAc), a nutraceutical that has various applications in healthcare Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information combinatorial fine-tuning of glucosamine-6-phosphate N-acetyltransferase (GNA1) and glutamine-fructose-6-phosphate aminotransferase (GlmS) expression by 5-terminus fusion engineering leads to overproduction of N-acetylglucosamine in Bacillus subtilis. Enhanced expression of GlmS is achieved at the transcriptional and translational levels by fusing an mRNA stabilizer to the 5'-terminus of GlmS gene. Under the control of GNA1 (fusion with cMyc tag and with the optimum RBS M-Rm) and GlmS (fusion with mRNA stabilizer DELTAermC+14/7A), the GlcNAc titer and yield in the shake flask increase to 18.5 g/l and 0.37 g GlcNAc/g glucose, which are 2.9fold and 2.3fold that of the control, respectively. Synthetic pathway fine-tuning method at the transcriptional and translational levels by combinatorial modulation of regulatory elements, including epitope tag, RBS sequence, and mRNA stabilizer, method evaluation, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + D-fructose 6-phosphate Bacillus subtilis
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?
L-glutamine + D-fructose 6-phosphate Bacillus subtilis 168
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 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P0CI73
-
-
Bacillus subtilis 168 P0CI73
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + D-fructose 6-phosphate
-
 Bacillus subtilis L-glutamate + D-glucosamine 6-phosphate
-
 ?
L-glutamine + D-fructose 6-phosphate
-
 Bacillus subtilis 168 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
BsglmS
-
 Bacillus subtilis
GlmS
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 Bacillus subtilis
glutamine-fructose-6-phosphate aminotransferase
-
 Bacillus subtilis

General Information

General Information Comment Organism
physiological function glucosamine-6-phosphate N-acetyltransferase (GNA1), catalyzing acetyl transfer from acetyl-coenzyme A to glucosamine-6-phosphate (GlcN-6P) and glutamine-fructose-6-phosphate aminotransferase (GlmS) catalyzing the formation of GlcN-6P from fructose-6-phosphate (Fru-6P), are two key enzymes in Bacillus subtilis for the bioproduction of N-acetylglucosamine (GlcNAc) Bacillus subtilis