Literature summary for 2.6.1.16 extracted from

Olchowy, J.; Kur, K.; Sachadyn, P.; Milewski, S.
Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli (2006), Protein Expr. Purif., 46, 309-315.

Search for more literature for 2.6.1.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) pLysS cells	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	D-fructose 6-phosphate	wild type enzyme, in in 25 mM potassium phosphate buffer at pH 7.0 and 37°C	Candida albicans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
79140	-	Superdex 200 HR 10/30 gel filtration	Candida albicans
80000	-	4 * 80000, SDS-PAGE	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-IDA agarose chromatography	Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.631	-	wild type enzyme, using D-fructose 6-phosphate as a substrate	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + D-fructose 6-phosphate	-	Candida albicans	L-glutamate + D-glucosamine 6-phosphate	-	ir

Subunits

Subunits	Comment	Organism
homotetramer	4 * 80000, SDS-PAGE	Candida albicans

Synonyms

Synonyms	Comment	Organism
GlcN-6-P synthase	-	Candida albicans
glucosamine-6-phosphate synthase	-	Candida albicans
L-glutamine: D-fructose-6-phosphate amidotransferase	-	Candida albicans

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Release 2023.1 (January 2023)