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Literature summary for 2.6.1.15 extracted from
- Kynurenine aminotransferase 3/glutamine transaminase L/cysteine conjugate beta-lyase 2 is a major glutamine transaminase in the mouse kidney (2016), Biochem. Biophys. Rep., 8, 234-241 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme expression in Escherichia coli, enzyme expression analysis by realtime RT-PCR | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methionine | 57% inhibition at 5 mM, competitive inhibition | Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
L-glutamine | recombinant enzyme, pH 9.0, 38°C | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + phenylpyruvate | Mus musculus | - |
2-oxoglutaramate + L-phenylalanine | - |
? | |
| selenomethionine + phenylpyruvate | Mus musculus | - |
2-oxo-gamma-methylselenobutyrate + L-phenylalanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q71RI9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| additional information | tissue localizations of KAT3/GTL/CCBL2, real-time RT-PCR enzyme expression analysis in tissues of female and male animals, overview. The enzyme is present in both liver and kidney of the mouse, but is much more abundant in the kidney than in the liver | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + indol-3-pyruvate | - |
Mus musculus | 2-oxoglutaramate + ? | - |
? | |
| L-glutamine + oxaloacetate | - |
Mus musculus | 2-oxoglutaramate + L-aspartate | - |
? | |
| L-glutamine + phenylpyruvate | - |
Mus musculus | 2-oxoglutaramate + L-phenylalanine | - |
? | |
| additional information | recombinant mouse KAT3/GTL selectively metabolizes selenomethionine to 2-oxo-gamma-methylselenobutyrate, KAT3/GTLis more efficient in transamination of both than is KAT1/GTK | Mus musculus | ? | - |
- |
|
| selenomethionine + phenylpyruvate | - |
Mus musculus | 2-oxo-gamma-methylselenobutyrate + L-phenylalanine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamine transaminase | - |
Mus musculus |
| glutamine transaminase L | - |
Mus musculus |
| GTL | - |
Mus musculus |
| KAT3/GTL/CCBL2 | - |
Mus musculus |
| More | see also EC 2.6.1.7 and EC 4.4.1.13 | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.67 | - |
L-glutamine | recombinant enzyme, pH 9.0, 38°C | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | kynurenine aminotransferase 3 (KAT3, EC 2.6.1.7) catalyzes the transamination of kynurenine to kynurenic acid, and is identical to cysteine conjugate beta-lyase 2 (CCBL2, EC 4.4.1.13) and glutamine transaminase L (GTL, EC 2.6.1.64) | Mus musculus |
| additional information | the enzyme is a key enzyme for studying the nephrotoxic mechanism of some xenobiotics and the formation of chemopreventive compounds in the mouse kidney | Mus musculus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.81 | - |
L-glutamine | recombinant enzyme, pH 9.0, 38°C | Mus musculus | |
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