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Literature summary for 2.6.1.13 extracted from

  • Hao, N.; Mu, J.; Hu, N.; Xu, S.; Shen, P.; Yan, M.; Li, Y.; Xu, L.
    Implication of ornithine acetyltransferase activity on L-ornithine production in Corynebacterium glutamicum (2016), Biotechnol. Appl. Biochem., 63, 15-21 .
    View publication on PubMed
Search for more literature for 2.6.1.13

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in Corynebacterium glutamincum strain 1006, subcloning in Escherichia coli strain DH5alpha Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information Corynebacterium glutamicum strain 1006 is engineered to overproduce L-ornithine as a major product by inactivating regulatory repressor argR gene and overexpressing argJ gene. A genome sequence analysis indicates that the argF gene encoding ornithine carbamoyltransferase in strain 1006 is mutated, resulting in the accumulation of a certain amount of L-ornithine (20.5 g/l). The OAT activity of Corynebacterium glutamicum mutant strain 1006DELTAargR-argJ is significantly greater than that of wild-type strain 1006, showing a very high conversion ratio of sugar to acid (0.396 g/g) Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
L-ornithine substrate inhibition Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + 2-oxoglutarate Corynebacterium glutamicum
-
 L-glutamate 5-semialdehyde + L-glutamate
-
 r
L-ornithine + 2-oxoglutarate Corynebacterium glutamicum 1006
-
 L-glutamate 5-semialdehyde + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum 1006
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + 2-oxoglutarate
-
 Corynebacterium glutamicum L-glutamate 5-semialdehyde + L-glutamate
-
 r
L-ornithine + 2-oxoglutarate
-
 Corynebacterium glutamicum 1006 L-glutamate 5-semialdehyde + L-glutamate
-
 r

Synonyms

Synonyms Comment Organism
OAT
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP Corynebacterium glutamicum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
 pH 7.5, 22°C, recombinant wild-type enzyme Corynebacterium glutamicum L-ornithine

General Information

General Information Comment Organism
metabolism the expression of the genes involved in the arginine operon (argCJBDFGH) for the control of the L-ornithine biosynthesis pathway that is regulated through the binding of ArgR to the so-called ARG operator sites preceding those relevant target genes Corynebacterium glutamicum
physiological function the Corynebacterium glutamicum ornithine acetyltransferase (OATase) ArgJ strongly influences the production of L-ornithine by OAT in Corynebacterium glutamicum Corynebacterium glutamicum