Literature summary for 2.6.1.13 extracted from

Ravi Kumar, H.; Ananda, S.; Devaraju, K.; Prakash, B.; Sampath Kumar, S.; Suresh Babu, S.; Ramachandraswamy, N.; Puttaraju, H.
A sensitive assay for ornithine amino transferase in rat brain mitochondria by ninhydrin method (2009), Indian J. Clin. Biochem., 24, 275-279.

Search for more literature for 2.6.1.13

Application

Application	Comment	Organism
diagnostics	the standardization of the enzyme kinetics can be extended to detect enzyme activity in patients of gyrate atrophy of choroid and retina with hyperornithinemia by studying the OAT activity	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.089	-	L-ornithine	in rat brain mitochondrial fraction, in absence of pyridoxal 5'-phosphate	Rattus norvegicus
5.089	-	L-ornithine	in rat brain mitochondrial fraction, in presence of 0.05 mM pyridoxal 5'-phosphate	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-ornithine + 2-oxoglutarate	Rattus norvegicus	-	DELTA1-pyrroline-5-carboxylate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-
heart	-	Rattus norvegicus	-
kidney	-	Rattus norvegicus	-
liver	-	Rattus norvegicus	-
lung	-	Rattus norvegicus	-
muscle	-	Rattus norvegicus	-
small intestine	-	Rattus norvegicus	-
spleen	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	Vmax in rat brain mitochondrial fraction is 0.565 nmol/min, in absence of pyridoxal 5'-phosphate	Rattus norvegicus
additional information	-	Vmax in rat brain mitochondrial fraction is 1.57 nmol/min, in presence of 0.05 mM pyridoxal 5'-phosphate	Rattus norvegicus
0.0052	-	muscle tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.0168	-	heart tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.02	-	brain tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.0225	-	spleen tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.0284	-	lung tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.0483	-	small intestine tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.0721	-	kidney tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus
0.1156	-	liver tissue, 35 mM L-ornithine, 5 mM 2-oxoglutarate and cofactor 0.05 mM pyridoxal 5'-phosphate in 50 mM Tris-HCl (pH-8.0)	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ornithine + 2-oxoglutarate	-	Rattus norvegicus	DELTA1-pyrroline-5-carboxylate + L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
OAT	-	Rattus norvegicus
ornithine aminotransferase	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Rattus norvegicus

General Information

General Information	Comment	Organism
metabolism	OAT plays a different role in arginine, ornithine and proline metabolism depending on the tissue and their physiological needs	Rattus norvegicus

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Release 2023.1 (January 2023)