General Stability | Organism |
---|---|
2-mercaptoethanol or DTT stabilizes | Manduca sexta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-canaline | binds covalently to enzyme forming a canaline-pyridoxal phosphate oxime, dialysis against free pyridoxal phosphate slowly reactivates the enzyme as the oxime is replaced by pyridoxal phosphate | Manduca sexta | |
N-ethylmaleimide | complete inactivation at 0.1 and 1 mM after 20 min | Manduca sexta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
L-ornithine | pH 8, 37°C | Manduca sexta | |
3.2 | - |
2-oxoglutarate | pH 8, 37°C | Manduca sexta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Manduca sexta |
148000 | 152000 | nondenaturing PAGE, gel filtration | Manduca sexta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
tobacco hornworm | - |
Purification (Comment) | Organism |
---|---|
- |
Manduca sexta |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
- |
Manduca sexta |
Storage Stability | Organism |
---|---|
-60°C, long-term storage is best as a pellet under liquid-saturated ammonium sulfate | Manduca sexta |
1°C, sharp loss in activity, especially in absence of free pyridoxal phosphate and mercaptans | Manduca sexta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | - |
Manduca sexta | DELTA1-pyrroline-5-carboxylate + L-glutamate | DL-pyrroline-5-carboxylate | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Manduca sexta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Manduca sexta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0333 | - |
2-oxoglutarate | - |
Manduca sexta | |
0.0333 | - |
L-ornithine | - |
Manduca sexta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Manduca sexta | |
pyridoxal 5'-phosphate | 4 mol of pyridoxal phosphate per mol of enzyme | Manduca sexta |