Protein Variants | Comment | Organism |
---|---|---|
A283I | mutant completely lacks activity | Bacillus subtilis |
A283K | mutant does not form a covalent adduct with chorismate and shows no activity in enzyme assays | Bacillus subtilis |
A283V | mutant shows marginal activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
NH3 | pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis | |
0.42 | - |
L-glutamate | pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P28820 | subunit PabB | - |
Bacillus subtilis 168 | P28820 | subunit PabB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-amino-4-deoxychorismate + NH3 | - |
Bacillus subtilis | 4-amino-4-deoxychorismate + NH3 | - |
? | |
(2S)-2-amino-4-deoxychorismate + NH3 | - |
Bacillus subtilis 168 | 4-amino-4-deoxychorismate + NH3 | - |
? | |
2 L-glutamine + 2 H2O | - |
Bacillus subtilis | 2 L-glutamate + 2 NH3 | - |
? | |
2 L-glutamine + 2 H2O | - |
Bacillus subtilis 168 | 2 L-glutamate + 2 NH3 | - |
? | |
chorismate + 2 L-glutamine + H2O | - |
Bacillus subtilis | 4-amino-4-deoxychorismate + 2 L-glutamate + ammonia | - |
? | |
chorismate + 2 L-glutamine + H2O | - |
Bacillus subtilis 168 | 4-amino-4-deoxychorismate + 2 L-glutamate + ammonia | - |
? | |
chorismate + NH3 | - |
Bacillus subtilis | (2S)-2-amino-4-deoxychorismate + H2O | - |
? | |
chorismate + NH3 | - |
Bacillus subtilis 168 | (2S)-2-amino-4-deoxychorismate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PabB | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NH3 | pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis | |
0.005 | - |
L-glutamate | pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | the PabA subunit acts successively on two molecules of L-glutamine, hydrolysing each to L-glutamate and ammonia (cf. EC 3.5.1.2, glutaminase). The ammonia molecules are channeled to the active site of PabB, which catalyses the formation of 4-amino-4-deoxychorismate from chorismate in two steps via the intermediate 2-amino-4-deoxychorismate. PabB forms a covalent adduct with chorismate | Bacillus subtilis |