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Literature summary for 2.6.1.12 extracted from

  • Koide, Y.; Honma, M.; Shimomura, T.
    L-alanine-alpha-keto acid aminotransferase of Pseudomonas sp. (1977), Agric. Biol. Chem., 41, 781-784.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
enzyme F-II
-

Purification (Commentary)

Purification (Comment) Organism
enzyme F-II Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxobutanoate
-
Pseudomonas sp. pyruvate + 2-aminobutanoate
-
r
L-alanine + 2-oxoglutarate at 2% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + L-glutamate
-
r
L-alanine + 2-oxohexanoate at 81% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + 2-aminohexanoate
-
r
L-alanine + 2-oxoisohexanoate at 8% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + L-leucine
-
r
L-alanine + 2-oxoisopentanoate at 10% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + L-valine
-
r
L-alanine + 2-oxopentanoate at 108% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + L-norvaline
-
r
L-alanine + glyoxylate 53% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + glycine
-
?
L-alanine + oxaloacetate at 16% of the activity with 2-oxobutanoate Pseudomonas sp. pyruvate + L-aspartate
-
r
pyruvate + L-2-aminobutanoate preferred amino donor Pseudomonas sp. L-alanine + 2-oxobutanoate
-
r
pyruvate + L-aspartate 4% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + oxaloacetate
-
?
pyruvate + L-glutamate 4% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 2-oxoglutarate
-
r
pyruvate + L-isoleucine 6% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 3-methyl-2-oxopentanoate
-
?
pyruvate + L-leucine 16% of the activity with L-2-aminobutanoate Pseudomonas sp. alanine + 4-methyl-2-oxopentanoate i.e. 2-oxoisohexanoate r
pyruvate + L-norleucine 31% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 2-oxohexanoate
-
?
pyruvate + L-norvaline 84% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 2-oxopentanoate
-
r
pyruvate + L-phenylalanine 4% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 2-oxo-3-phenylpropanoate
-
?
pyruvate + L-threonine 3% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 3-hydroxy-2-oxobutanoate
-
?
pyruvate + L-valine 8% of the activity with L-2-aminobutanoate Pseudomonas sp. L-alanine + 3-methyl-2-oxobutanoate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas sp.