Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLys cells | Capsicum chinense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + vanillin | Capsicum chinense | - |
pyruvate + vanillylamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum chinense | O82521 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Capsicum chinense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethylamine + 4-hydroxybenzaldehyde | about 20% activity compared to pyruvate | Capsicum chinense | ? | - |
r | |
(S)-1-phenylethylamine + oxaloacetate | about 90% activity compared to pyruvate | Capsicum chinense | acetophenone + ? | - |
r | |
(S)-1-phenylethylamine + pyruvate | 100% activity | Capsicum chinense | acetophenone + ? | - |
r | |
L-alanine + vanillin | - |
Capsicum chinense | pyruvate + vanillylamine | - |
r | |
additional information | the enzyme has no activity with (R)-1-phenylethylamine. The enzyme shows no activity with succinate, acetone and 2-oxoglutarate as co-substrate | Capsicum chinense | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 57500, His-tagged enzyme, SDS-PAGE | Capsicum chinense |
Synonyms | Comment | Organism |
---|---|---|
PAMT | - |
Capsicum chinense |
VAMT | - |
Capsicum chinense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Capsicum chinense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 50% activity at pH 6.0-9.0 | Capsicum chinense |