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Literature summary for 2.6.1.11 extracted from

  • Rajaram, V.; Ratna Prasuna, P.; Savithri, H.S.; Murthy, M.R.
    Structure of biosynthetic N-acetylornithine aminotransferase from Salmonella typhimurium: studies on substrate specificity and inhibitor binding (2008), Proteins, 70, 429-441.
    View publication on PubMed
Search for more literature for 2.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
in unliganded form and in complex with gabaculine or L-gutamate, diffration to 1.9 A resolution. The structure of unliganded enzyme shows significant electron density for pyridoxal 5'-phosphate in subunits A which can be attributed to the ordering of the loop Lak-bm in subunit B Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
gabaculine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
NaCl 200 mM, 25% residual activity Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 N2-Acetyl-L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.64
-
 L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + 2-oxoglutarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-glutamate-gamma-semialdehyde + L-glutamate
-
 ?
N2-acetyl-L-ornithine + 2-oxoglutarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium N-acetyl-L-glutamate-gamma-semialdehyde + L-glutamate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.82
-
 L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium
1.55
-
 N2-Acetyl-L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 about 50% of maximal activity Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.007
-
 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium gabaculine