Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
GDP-4-dehydro-6-deoxy-alpha-D-mannose | pH 8, °C, His-tag RfbE fusion protein | Vibrio cholerae serotype O1 | |
0.1 | - |
L-glutamate | pH 8, 37°C, His-tag RfbE fusion protein | Vibrio cholerae serotype O1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration is 20 mM | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40900 | - |
4 * 40900, SDS-PAGE | Vibrio cholerae serotype O1 |
170000 | - |
gel filtration | Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate | Vibrio cholerae serotype O1 | - |
GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 8 | GDP-4-dehydro-6-deoxy-alpha-D-mannose, pH 8, 37°C, His-tag RfbE fusion protein | Vibrio cholerae serotype O1 |
42 | - |
L-glutamate, pH 8, 37°C, His-tag RfbE fusion protein | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate | - |
Vibrio cholerae serotype O1 | GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40900, SDS-PAGE | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase | - |
Vibrio cholerae serotype O1 |
RfbE | - |
Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | essential for catalysis of ther aminotransferase reaction | Vibrio cholerae serotype O1 |