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Literature summary for 2.6.1.102 extracted from

  • Albermann, C.; Piepersberg, W.
    Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine (2001), Glycobiology, 11, 655-661.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 8, °C, His-tag RfbE fusion protein Vibrio cholerae serotype O1
0.1
-
L-glutamate pH 8, 37°C, His-tag RfbE fusion protein Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration is 20 mM Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40900
-
4 * 40900, SDS-PAGE Vibrio cholerae serotype O1
170000
-
gel filtration Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate Vibrio cholerae serotype O1
-
GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8 GDP-4-dehydro-6-deoxy-alpha-D-mannose, pH 8, 37°C, His-tag RfbE fusion protein Vibrio cholerae serotype O1
42
-
L-glutamate, pH 8, 37°C, His-tag RfbE fusion protein Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate
-
Vibrio cholerae serotype O1 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40900, SDS-PAGE Vibrio cholerae serotype O1

Synonyms

Synonyms Comment Organism
GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase
-
Vibrio cholerae serotype O1
RfbE
-
Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Vibrio cholerae serotype O1

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate essential for catalysis of ther aminotransferase reaction Vibrio cholerae serotype O1