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Literature summary for 2.6.1.1 extracted from

  • Jansen, R.S.; Mandyoli, L.; Hughes, R.; Wakabayashi, S.; Pinkham, J.T.; Selbach, B.; Guinn, K.M.; Rubin, E.J.; Sacchettini, J.C.; Rhee, K.Y.
    Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis (2020), Nat. Commun., 11, 1960 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.34
-
 L-aspartate at pH 7.4 and 37°C Mycobacterium tuberculosis
29.4
-
 kynurenine at pH 7.4 and 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Mycobacterium tuberculosis
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Mycobacterium tuberculosis H37Rv
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O69689
-
-
Mycobacterium tuberculosis H37Rv O69689
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kynurenine + 2-oxoglutarate
-
 Mycobacterium tuberculosis ?
-
 ?
kynurenine + 2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
L-aspartate + 2-oxoglutarate
-
 Mycobacterium tuberculosis oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv oxaloacetate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
aspartate aminotransferase
-
 Mycobacterium tuberculosis
AspAT
-
 Mycobacterium tuberculosis
Rv3722c
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
136
-
 L-aspartate at pH 7.4 and 37°C Mycobacterium tuberculosis
198
-
 kynurenine at pH 7.4 and 37°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
malfunction enzyme deficiency leads to virulence attenuation in macrophages and mice Mycobacterium tuberculosis
physiological function the enzyme is essential for virulence in macrophages and mice Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.73
-
 kynurenine at pH 7.4 and 37°C Mycobacterium tuberculosis
58.3
-
 L-aspartate at pH 7.4 and 37°C Mycobacterium tuberculosis