Literature summary for 2.6.1.1 extracted from

Andreessen, C.; Gerlt, V.; Steinbuechel, A.
Conversion of cysteine to 3-mercaptopyruvic acid by bacterial aminotransferases (2017), Enzyme Microb. Technol., 99, 38-48 .

Search for more literature for 2.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Cupriavidus necator
expressed in Escherichia coli BL21(DE3) cells	Shimwellia blattae
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli
expressed in Escherichia coli BL21(DE3) cells	Advenella mimigardefordensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.039	-	L-aspartate	at pH 7.4 and 30°C	Advenella mimigardefordensis
0.108	-	L-aspartate	at pH 7.4 and 30°C	Escherichia coli
8.57	-	L-cysteine	at pH 7.4 and 30°C	Advenella mimigardefordensis
20.62	-	L-cysteine	at pH 7.4 and 30°C	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	Cupriavidus necator	-	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	Shimwellia blattae	-	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	Escherichia coli	-	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	Advenella mimigardefordensis	-	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	Advenella mimigardefordensis DPN7	-	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	Escherichia coli MG1655	-	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	Shimwellia blattae ATCC 33430	-	oxaloacetate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Advenella mimigardefordensis	W0PFQ7	-	-
Advenella mimigardefordensis DPN7	W0PFQ7	-	-
Cupriavidus necator	-	-	-
Escherichia coli	P00509	-	-
Escherichia coli MG1655	P00509	-	-
Shimwellia blattae	-	-	-
Shimwellia blattae ATCC 33430	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His SpinTrap column chromatography	Cupriavidus necator
His SpinTrap column chromatography	Shimwellia blattae
His SpinTrap column chromatography	Escherichia coli
His SpinTrap column chromatography	Advenella mimigardefordensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	-	Cupriavidus necator	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	-	Shimwellia blattae	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	-	Escherichia coli	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	-	Advenella mimigardefordensis	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	-	Advenella mimigardefordensis DPN7	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	-	Escherichia coli MG1655	oxaloacetate + L-glutamate	-	?
L-aspartate + 2-oxoglutarate	-	Shimwellia blattae ATCC 33430	oxaloacetate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Cupriavidus necator	3-mercaptopyruvate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Shimwellia blattae	3-mercaptopyruvate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Escherichia coli	3-mercaptopyruvate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Advenella mimigardefordensis	3-mercaptopyruvate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Advenella mimigardefordensis DPN7	3-mercaptopyruvate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Escherichia coli MG1655	3-mercaptopyruvate + L-glutamate	-	?
L-cysteine + 2-oxoglutarate	-	Shimwellia blattae ATCC 33430	3-mercaptopyruvate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Cupriavidus necator
?	x * 40000, SDS-PAGE	Shimwellia blattae
?	x * 40000, SDS-PAGE	Escherichia coli
?	x * 40000, SDS-PAGE	Advenella mimigardefordensis
?	x * 43900, calculated from amino acid sequence	Advenella mimigardefordensis
?	x * 43600, calculated from amino acid sequence	Escherichia coli
?	x * 43800, calculated from amino acid sequence	Shimwellia blattae
?	x * 43200, calculated from amino acid sequence	Cupriavidus necator

Synonyms

Synonyms	Comment	Organism
AAT	-	Cupriavidus necator
AAT	-	Shimwellia blattae
AAT	-	Escherichia coli
AAT	-	Advenella mimigardefordensis
aspartate aminotransferase	-	Cupriavidus necator
aspartate aminotransferase	-	Shimwellia blattae
aspartate aminotransferase	-	Escherichia coli
aspartate aminotransferase	-	Advenella mimigardefordensis
aspC	-	Cupriavidus necator
aspC	-	Shimwellia blattae
aspC	-	Escherichia coli
aspC	-	Advenella mimigardefordensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Escherichia coli
40	-	-	Advenella mimigardefordensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
19.66	-	L-aspartate	at pH 7.4 and 30°C	Advenella mimigardefordensis
24.43	-	L-aspartate	at pH 7.4 and 30°C	Escherichia coli
40.64	-	L-cysteine	at pH 7.4 and 30°C	Escherichia coli
43.85	-	L-cysteine	at pH 7.4 and 30°C	Advenella mimigardefordensis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Cupriavidus necator
pyridoxal 5'-phosphate	-	Shimwellia blattae
pyridoxal 5'-phosphate	-	Escherichia coli
pyridoxal 5'-phosphate	-	Advenella mimigardefordensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Shimwellia blattae	calculated from amino acid sequence	-	5.4
Escherichia coli	calculated from amino acid sequence	-	5.5
Advenella mimigardefordensis	calculated from amino acid sequence	-	6
Cupriavidus necator	calculated from amino acid sequence	-	6.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2	-	L-cysteine	at pH 7.4 and 30°C	Escherichia coli
5.1	-	L-cysteine	at pH 7.4 and 30°C	Advenella mimigardefordensis
226.2	-	L-aspartate	at pH 7.4 and 30°C	Escherichia coli
504.1	-	L-aspartate	at pH 7.4 and 30°C	Advenella mimigardefordensis

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Release 2023.1 (January 2023)