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Literature summary for 2.6.1.1 extracted from

  • Marciano, D.; Llorente, C.; Maugeri, D.A.; de la Fuente, C.; Opperdoes, F.; Cazzulo, J.J.; Nowicki, C.
    Biochemical characterization of stage-specific isoforms of aspartate aminotransferases from Trypanosoma cruzi and Trypanosoma brucei (2008), Mol. Biochem. Parasitol., 161, 12-20.
    View publication on PubMed
Search for more literature for 2.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His-tagged protein Trypanosoma cruzi
expression in Escherichia coli, His-tagged protein Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 L-phenylalanine pH 7.5, 37°C Trypanosoma cruzi
1.5
-
 2-oxoglutarate pH 7.5, 37°C Trypanosoma brucei
1.5
-
 L-tyrosine pH 7.5, 37°C Trypanosoma cruzi
1.6
-
 L-tyrosine pH 7.5, 37°C Trypanosoma brucei
2.1
-
 L-aspartate pH 7.5, 37°C Trypanosoma cruzi
2.3
-
 L-tryptophan pH 7.5, 37°C Trypanosoma cruzi
3.2
-
 2-oxoglutarate pH 7.5, 37°C Trypanosoma cruzi
6.6
-
 L-phenylalanine pH 7.5, 37°C Trypanosoma brucei
6.8
-
 L-aspartate pH 7.5, 37°C Trypanosoma brucei
6.9
-
 L-methionine pH 7.5, 37°C Trypanosoma cruzi
26
-
 L-aspartate pH 7.5, 37°C Trypanosoma brucei
37
-
 L-aspartate pH 7.5, 37°C Trypanosoma cruzi
56
-
 L-methionine pH 7.5, 37°C Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol enzyme is constitutively expressed Trypanosoma brucei 5829
-
cytosol enzyme is specifically induced in the mammalian stages of Trypanosoma cruzi Trypanosoma cruzi 5829
-
mitochondrion enzyme is constitutively expressed Trypanosoma cruzi 5739
-
mitochondrion enzyme is down-regulated in bloddstream forms of Trypanosoma brucei Trypanosoma brucei 5739
-

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q964F0 mitochondrial isoform
-
Trypanosoma brucei Q964F1 cytosolic isoform
-
Trypanosoma cruzi Q4D080 cytosolic isoform
-
Trypanosoma cruzi Q4D1Q4 mitochondrial isoform
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Trypanosoma cruzi oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Trypanosoma brucei oxaloacetate + L-glutamate
-
 ?
L-methionine + 2-oxoglutarate
-
 Trypanosoma cruzi L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
L-methionine + 2-oxoglutarate
-
 Trypanosoma brucei L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Trypanosoma cruzi 2-oxo-3-phenylpropionic acid + L-glutamate
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Trypanosoma brucei 2-oxo-3-phenylpropionic acid + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Trypanosoma cruzi 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Trypanosoma brucei 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Trypanosoma cruzi 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Trypanosoma brucei 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
 ?
additional information the cytosolic isoform displays broad substrate specificity Trypanosoma cruzi ?
-
 ?
additional information the cytosolic isoform displays broad substrate specificity Trypanosoma brucei ?
-
 ?
additional information the mitochondrial isoform is highly specific for substrates L-aspartate and 2-oxoglutarate Trypanosoma brucei ?
-
 ?
additional information the mitochondrial isoform is highly specific for substrates L-aspartate and 2-oxoglutarate Trypanosoma cruzi ?
-
 ?