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Literature summary for 2.6.1.1 extracted from

  • de la Torre, F.; Suarez, M.F.; Santis, L.; Canovas, F.M.
    The aspartate aminotransferase family in conifers: biochemical analysis of a prokaryotic-type enzyme from maritime pine (2007), Tree Physiol., 27, 1283-1291.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pinus pinaster
expression in Escherichia coli Pinus pinaster

Organism

Organism UniProt Comment Textmining
Pinus pinaster
-
-
-
Pinus pinaster
-
prokaryotic-type isoform
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography and DEAE Sephacel column chromatography Pinus pinaster
recombinant protein, rapid protocol for purification Pinus pinaster

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Pinus pinaster
-
root
-
Pinus pinaster
-
stem
-
Pinus pinaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.87
-
in 50 mM Tris-HCl pH 7.8 Pinus pinaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Pinus pinaster oxaloacetate + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
AAT
-
Pinus pinaster
aspartate aminotransferase
-
Pinus pinaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60 highest catalytic activity Pinus pinaster

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60
-
Pinus pinaster

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
presence of pyridoxal 5'-phosphate significantly increases the thermostability Pinus pinaster
70
-
moderately thermostable, retaining about 50% of its activity after incubation at 70°C for 5-10 min Pinus pinaster
70
-
5-10 min, 50% residual activity Pinus pinaster

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Pinus pinaster