Cloned (Comment) | Organism |
---|---|
isolation of genomic DNA and construction of a genetic library, cloning of gene PhaspC, DNA and amino acid sequence determination, overexpression in Escherichia coli strain TY103 | Pseudoalteromonas haloplanktis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant enzyme, high temperature dependence of Km | Pseudoalteromonas haloplanktis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43776 | - |
x * 43776, recombinant enzyme, DNA sequence determination and mass spectrometry | Pseudoalteromonas haloplanktis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Pseudoalteromonas haloplanktis | - |
oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | Pseudoalteromonas haloplanktis TAC 125 | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas haloplanktis | - |
- |
- |
Pseudoalteromonas haloplanktis TAC 125 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of native enzyme, purification of recombinant enzyme from Escherichia coli | Pseudoalteromonas haloplanktis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudoalteromonas haloplanktis |
12.92 | - |
recombinant enzyme in Escherichia coli cells | Pseudoalteromonas haloplanktis |
137.7 | - |
purified recombinant enzyme | Pseudoalteromonas haloplanktis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Pseudoalteromonas haloplanktis | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Pseudoalteromonas haloplanktis TAC 125 | oxaloacetate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43776, recombinant enzyme, DNA sequence determination and mass spectrometry | Pseudoalteromonas haloplanktis |
More | structure modeling, subunit organization | Pseudoalteromonas haloplanktis |
Synonyms | Comment | Organism |
---|---|---|
AspAT | - |
Pseudoalteromonas haloplanktis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudoalteromonas haloplanktis |
34 | - |
optimal temperature of recombinant enzyme in Escherichia coli in vivo | Pseudoalteromonas haloplanktis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 63 | half-maximal activity at 35°C and 63°C | Pseudoalteromonas haloplanktis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
t1/2: 6.8 min | Pseudoalteromonas haloplanktis |
50 | 65 | recombinant enzyme, transition temperature | Pseudoalteromonas haloplanktis |
65.5 | - |
recombinant enzyme, melting temperature | Pseudoalteromonas haloplanktis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudoalteromonas haloplanktis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudoalteromonas haloplanktis |