Crystallization (Comment) | Organism |
---|---|
purified mutant V39L, hanging drop method, room temperature, protein solution: 12.5 mg/ml, 20 mM sodium phosphate, pH 7.5, 30 mM maleate, 0.4 M sodium chloride, 12.5% w/v polyethylene glycol 4000, reservoir solution: 23-25% w/v polyethylene glycol 4000, 0.8 M sodium chloride, 20 mM sodium phosphate, pH 7.5, X-ray diffraction structure determination | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
V39L | crystal structure analysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant V39L | - |
Purification (Comment) | Organism |
---|---|
mutant V39L | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate | bi bi ping pong reaction kinetic | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
108 | - |
mutant V39L | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 0.7-0.9 mg/ml protein in 20 to 40 mM sodium phosphate buffer, pH 7.0, 0.01 mM pyridoxal phosphate, 1 mM 2-oxoglutarate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r |