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Literature summary for 2.6.1.1 extracted from

  • Jäger, J.; Köhler, E.; Tucker, P.; Sauder, U.; Housley-Markovic, Z.; Fotheringham, I.; Edwards, M.; Hunter, M.; Kirschner, K.; Jansonius, J.N.
    Crystallization and preliminary X-ray studies of an aspartate aminotransferase mutant from Escherichia coli (1989), J. Mol. Biol., 209, 499-501.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified mutant V39L, hanging drop method, room temperature, protein solution: 12.5 mg/ml, 20 mM sodium phosphate, pH 7.5, 30 mM maleate, 0.4 M sodium chloride, 12.5% w/v polyethylene glycol 4000, reservoir solution: 23-25% w/v polyethylene glycol 4000, 0.8 M sodium chloride, 20 mM sodium phosphate, pH 7.5, X-ray diffraction structure determination Escherichia coli

Protein Variants

Protein Variants Comment Organism
V39L crystal structure analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Escherichia coli
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
mutant V39L
-

Purification (Commentary)

Purification (Comment) Organism
mutant V39L Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108
-
mutant V39L Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, 0.7-0.9 mg/ml protein in 20 to 40 mM sodium phosphate buffer, pH 7.0, 0.01 mM pyridoxal phosphate, 1 mM 2-oxoglutarate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Escherichia coli oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate
-
Escherichia coli oxaloacetate + L-glutamate
-
r