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Literature summary for 2.6.1.1 extracted from

  • Owen, T.G.; Hochachka, P.W.
    Purification and properties of dolphin muscle aspartate and alanine transaminases and thier possible roles in the energy metabolism of diving mammals (1974), Biochem. J., 143, 541-553.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate oxoglutarate production; substrate inhibition at high concentration dolphin
L-glutamate forward reaction, substrate L-aspartate dolphin
malate isozymes Asp-DEAE-1 and Asp-DEAE-2 dolphin
oxaloacetate substrate inhibition dolphin
succinate forward reaction dolphin

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
dolphin

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoenzyme dolphin 5829
-
mitochondrion mitochondrial isoenzyme dolphin 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate dolphin role in energy metabolism of diving animals oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
dolphin
-
cytosolic and mitochondrial isozyme
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic and mitochondrial isozymes dolphin

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
dolphin
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
purified cytosolic isozyme dolphin
228
-
purified mitochondrial isozyme dolphin

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
dolphin oxaloacetate + L-glutamate
-
r
L-aspartate + 2-oxoglutarate role in energy metabolism of diving animals dolphin oxaloacetate + L-glutamate
-
?
L-glutamate + 2-oxoglutarate L-glutamate is best amino acid donor dolphin 2-oxoglutarate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
dolphin 2-oxo-3-phenylpropionic acid + L-glutamate
-
ir
L-tyrosine + 2-oxoglutarate
-
dolphin 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 7.3 mitochondrial enzyme dolphin
7.3 8.3 cytosolic enzyme dolphin

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.3 pH 6.8: 76% of activity maximum, pH 7.3-8.3: activity maximum, supernatant enzyme dolphin