Any feedback?
Literature summary for 2.5.1.B43 extracted from
- The photoheterotrophic growth of bacteriochlorophyll synthase-deficient mutant of Rhodobacter sphaeroides is restored by I44F mutant chlorophyll synthase of Synechocystis sp. PCC 6803 (2016), J. Microbiol. Biotechnol., 26, 959-966.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bchG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Cereibacter sphaeroides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F28I | naturally occuring mutation, the mutant shows chlorophyll synthase, EC 2.5.1.62, ChlG, activity | Cereibacter sphaeroides |
| additional information | a BchG mutant Rhodobacter sphaeroides BG1 strain is photosynthetically incompetent. Expression of gene chlG from Synechocystis sp. PCC 6803, encoding a chlorophyll synthase I44F mutant, EC 2.5.1.62, rescues the photoheterotrophical growth of the mutant cells. The Synechocystis mutated enzyme ChlGI44F shows phytyl-dependent bacteriohchlorophyll a synthase activity | Cereibacter sphaeroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
bacteriochlorophyllide a | pH 7.6, 30°C, recombinant wild-type enzyme | Cereibacter sphaeroides |  |
| 0.16 | - |
bacteriochlorophyllide a | pH 7.6, 30°C, recombinant enzyme mutant R28I | Cereibacter sphaeroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Cereibacter sphaeroides | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bacteriochlorophyllide a + phytyl diphosphate | Cereibacter sphaeroides | - |
bacteriochlorophyll a + diphosphate | - |
? | |
| additional information | Cereibacter sphaeroides | bacteriochlorophyll synthase has a high degree of substrate specificity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | Q9Z5D6 | gene bchG | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bacteriochlorophyllide a + phytyl diphosphate | - |
Cereibacter sphaeroides | bacteriochlorophyll a + diphosphate | - |
? | |
| additional information | bacteriochlorophyll synthase has a high degree of substrate specificity | Cereibacter sphaeroides | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bchG | - |
Cereibacter sphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Cereibacter sphaeroides |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the predicted sequence of ChlG of Synechocystis sp. PCC 6803 is considerably similar (35% identity) to that of Rhodobacter sphaeroides BchG, each enzyme has a high level of substrate specificity to distinguish its own substrate from the other. But the BchG activity of Synechocystis chlorophyll synthase mutant ChlGI44F and the ChlG activity of bacteriochlorophyll synthase mutant BchGF28I suggest that ChlG/EC 2.5.1.62 and BchG/EC 2.5.1.B43 are evolutionarily related enzymes, cf. EC 2.5.1.133 | Cereibacter sphaeroides |
| additional information | residue F28 is important for the substrate specificities of the enzyme | Cereibacter sphaeroides |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
