Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a His8-tagged protein | Sclerotinia sclerotiorum |
expression in Escherichia coli as a His8-tagged protein | Botrytis cinerea |
expression in Escherichia coli as a His8-tagged protein | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,7-dihydroxynaphthalene | 20 mM, almost complete inhibition | Aspergillus terreus | |
2,7-dihydroxynaphthalene | 20 mM, almost complete inhibition | Sclerotinia sclerotiorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.324 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Aspergillus terreus | |
0.325 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Aspergillus terreus | |
0.59 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Sclerotinia sclerotiorum | |
0.996 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Botrytis cinerea | |
1.01 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Botrytis cinerea | |
1.232 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Sclerotinia sclerotiorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | addition of MgCl2 to the incubation mixture increases the reaction velocity. The optimal concentration of MgCl2 is 10 mM, leading to a 2.4-fold increase of product formation. The enzyme shows catalytic activity in the complete absence of Mg2+ or any other divalent cation | Aspergillus terreus | |
Mg2+ | the enzyme is active in the absence of Mg2+, but addition of 10 mM MgCl2 increases reaction velocity by a factor of 2.6 | Sclerotinia sclerotiorum | |
Mg2+ | the enzyme is active in the absence of Mg2+, but addition of 10 mM MgCl2 increases reaction velocity by a factor of 2.6 | Botrytis cinerea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | Q0CZR3 | - |
- |
Aspergillus terreus NIHH 2624 | Q0CZR3 | - |
- |
Botrytis cinerea | - |
- |
- |
Botrytis cinerea B05.10 | - |
- |
- |
Sclerotinia sclerotiorum | - |
- |
- |
Sclerotinia sclerotiorum 1980 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sclerotinia sclerotiorum |
- |
Botrytis cinerea |
- |
Aspergillus terreus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene = diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | Friedel-Crafts alkylation | Aspergillus terreus |
Storage Stability | Organism |
---|---|
-80°C, 2 months, less than 5% loss of activity | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate | Sclerotinia sclerotiorum | diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate | Botrytis cinerea | diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate | Aspergillus terreus | diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate | Sclerotinia sclerotiorum 1980 | diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate | Aspergillus terreus NIHH 2624 | diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | the enzyme is specific for dimethylallyl diphosphate. No activity with geranyl diphosphate or isopentenyl diphosphate | Botrytis cinerea B05.10 | diphosphate + 2,7-dihydroxy-3-dimethylallylnaphthalene | - |
? | |
additional information | significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate | Sclerotinia sclerotiorum | ? | - |
? | |
additional information | significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate | Aspergillus terreus | ? | - |
? | |
additional information | significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid or 4-hydroxybenzoate as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate | Botrytis cinerea | ? | - |
? | |
additional information | significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate | Sclerotinia sclerotiorum 1980 | ? | - |
? | |
additional information | significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin, or 4-hydroxybenzoic acid. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate | Aspergillus terreus NIHH 2624 | ? | - |
? | |
additional information | significantly lower level of prenylation activity with dihydrophenazine-1-carboxylic acid or 4-hydroxybenzoate as aromatic substrate, and only traces of products are detected with aspulvinone E, flaviolin. No product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate | Botrytis cinerea B05.10 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PtfAt | - |
Aspergillus terreus |
PtfBf | - |
Botrytis cinerea |
PtfSs | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sclerotinia sclerotiorum |
30 | - |
assay at | Botrytis cinerea |
30 | - |
assay at | Aspergillus terreus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Aspergillus terreus | |
0.026 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Aspergillus terreus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.1 | 9.4 | pH 8.1: about 50% of maximal activity, pH 9.4: about 50% of maximal activity | Aspergillus terreus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Botrytis cinerea | |
0.005 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Botrytis cinerea | |
0.022 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Sclerotinia sclerotiorum | |
0.022 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Sclerotinia sclerotiorum | |
0.08 | - |
dimethylallyl diphosphate | pH 8.7, 30°C | Aspergillus terreus | |
0.08 | - |
2,7-dihydroxynaphthalene | pH 8.7, 30°C | Aspergillus terreus |