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Literature summary for 2.5.1.97 extracted from
- Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase (2005), J. Biol. Chem., 280, 35922-35928.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Campylobacter jejuni |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
phosphoenolpyruvate | pH 7.4, 25°C | Campylobacter jejuni |  |
| 0.0095 | - |
2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose | pH 7.4, 25°C | Campylobacter jejuni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. Addition of 10 mM Mg2+ results in 30% of the activity obtained with 10 mM Co2+ | Campylobacter jejuni | |
| Co2+ | pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. The highest values are observed in the presence of Mn2+ and Co2+ (10 mM) | Campylobacter jejuni | |
| Mg2+ | pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. Addition of 10 mM Mg2+ results in 48% of the activity obtained with 10 mM Co2+ | Campylobacter jejuni | |
| Mn2+ | pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. The highest values are observed in the presence of Mn2+ and Co2+ (10 mM) | Campylobacter jejuni | |
| Ni2+ | pseudaminic acid synthase requires the presence of a divalent metal ion for catalysis. Addition of 10 mM Mg2+ results in 26% of the activity obtained with 10 mM Co2+ | Campylobacter jejuni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38647 | - |
x * 38647, calculated from sequence | Campylobacter jejuni |
| 38670 | - |
x * 38670, electrospray ionization-mass spectrometry, SDS-PAGE | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O | Campylobacter jejuni | pseudaminic acid biosynthesis | phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | N-acetyl-D-mannosamine can not replace 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose as a substrate indicating that the enzyme does not possess detectable sialic acid synthase activity | Campylobacter jejuni | ? | - |
? | |
| phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O | pseudaminic acid biosynthesis | Campylobacter jejuni | phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid | - |
? | |
| phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O | phosphoenolpyruvate attacks the si-face of the aldehyde of 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose to generate an (S)-configuration at C-4. The proposed catalytic mechanism of pseudaminic acid synthase is the attack of the C-3 of PEP to the aldehyde of the open chain form of 6-deoxy-AltdiNAc and an attack of water to the C-2 of phosphoenolpyruvate to form a tetrahedral intermediate. The tetrahedral intermediate then collapses to release inorganic phosphate and form the open chain form of pseudaminic acid that cyclizes to the pyranose form in solution | Campylobacter jejuni | phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38647, calculated from sequence | Campylobacter jejuni |
| ? | x * 38670, electrospray ionization-mass spectrometry, SDS-PAGE | Campylobacter jejuni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NeuB3 | - |
Campylobacter jejuni |
| PseG | - |
Campylobacter jejuni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose | pH 7.4, 25°C | Campylobacter jejuni | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Campylobacter jejuni |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Campylobacter jejuni and Campylobacter coli are the main causes of bacterial diarrhea worldwide. In all of these pathogenic organisms, the flagellin proteins are heavily glycosylated with pseudaminic acid (the product of the PseI catalyzed reaction). The presence of 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid is required for the proper development of the flagella and is thereby necessary for motility in, and invasion of, the host | Campylobacter jejuni |
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