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Literature summary for 2.5.1.94 extracted from
- A coupled chlorinase-fluorinase system with a high efficiency of trans-halogenation and a shared substrate tolerance (2018), Chem. Commun. (Camb.), 54, 9458-9461 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | deveopment of a coupled chlorinase-fluorinase system for rapid transhalogenation. The chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine in up to 91.6% yield in 1 h | Streptomyces albulus |
| synthesis | deveopment of a coupled chlorinase-fluorinase system for rapid transhalogenation. The chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine in up to 91.6% yield in 1 h | Umezawaea tangerina |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00248 | - |
S-adenosyl-L-methionine | isoform ClA2, at pH 7.8 and 37°C | uncultured bacterium |  |
| 0.0025 | - |
S-adenosyl-L-methionine | pH not specified in the publication, temperature not specified in the publication | Umezawaea tangerina | |
| 0.00945 | - |
5-deoxy-5-chloroadenosine | isoform ClA2, at pH 7.8 and 37°C | uncultured bacterium | |
| 0.0095 | - |
5-deoxy-5'-chloroadenosine | pH not specified in the publication, temperature not specified in the publication | Umezawaea tangerina | |
| 0.01398 | - |
5-deoxy-5-chloroadenosine | isoform ClA1, at pH 7.8 and 37°C | uncultured bacterium | |
| 0.014 | - |
5-deoxy-5'-chloroadenosine | pH not specified in the publication, temperature not specified in the publication | Streptomyces albulus | |
| 0.01569 | - |
S-adenosyl-L-methionine | isoform ClA1, at pH 7.8 and 37°C | uncultured bacterium | |
| 0.0157 | - |
S-adenosyl-L-methionine | pH not specified in the publication, temperature not specified in the publication | Streptomyces albulus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-deoxy-5-chloroadenosine + L-methionine | uncultured bacterium | most efficient reaction | S-adenosyl-L-methionine + chloride | - |
r | |
| S-adenosyl-L-methionine + chloride | uncultured bacterium | - |
5-deoxy-5-chloroadenosine + L-methionine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces albulus | - |
- |
- |
| Umezawaea tangerina | - |
- |
- |
| Umezawaea tangerina NRRL B-24463 | - |
- |
- |
| uncultured bacterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TALON metal affinity resin column chromatography | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride | - |
Streptomyces albulus | 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine | - |
r | |
| 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride | - |
Umezawaea tangerina | 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine | - |
r | |
| 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride | - |
Umezawaea tangerina NRRL B-24463 | 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine | - |
r | |
| 5-deoxy-5'-chloroadenosine + L-methionine | - |
Streptomyces albulus | S-adenosyl-L-methionine + chloride | - |
r | |
| 5-deoxy-5'-chloroadenosine + L-methionine | - |
Umezawaea tangerina | S-adenosyl-L-methionine + chloride | - |
r | |
| 5-deoxy-5'-chloroadenosine + L-methionine | - |
Umezawaea tangerina NRRL B-24463 | S-adenosyl-L-methionine + chloride | - |
r | |
| 5-deoxy-5-chloroadenosine + L-methionine | most efficient reaction | uncultured bacterium | S-adenosyl-L-methionine + chloride | - |
r | |
| S-adenosyl-L-methionine + chloride | - |
uncultured bacterium | 5-deoxy-5-chloroadenosine + L-methionine | - |
r | |
| S-adenosyl-L-methionine + chloride | - |
Streptomyces albulus | 5-deoxy-5'-chloroadenosine + L-methionine | - |
r | |
| S-adenosyl-L-methionine + chloride | - |
Umezawaea tangerina | 5-deoxy-5'-chloroadenosine + L-methionine | - |
r | |
| S-adenosyl-L-methionine + chloride | - |
Umezawaea tangerina NRRL B-24463 | 5-deoxy-5'-chloroadenosine + L-methionine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * about 30000-35000, SDS-PAGE | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cla1 | - |
uncultured bacterium |
| Cla1 | - |
Streptomyces albulus |
| ClA2 | - |
uncultured bacterium |
| ClA2 | - |
Umezawaea tangerina |
| SAM-dependent chlorinase | - |
uncultured bacterium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
S-adenosyl-L-methionine | pH not specified in the publication, temperature not specified in the publication | Umezawaea tangerina | |
| 0.011 | - |
S-adenosyl-L-methionine | isoform ClA2, at pH 7.8 and 37°C | uncultured bacterium | |
| 0.078 | - |
S-adenosyl-L-methionine | pH not specified in the publication, temperature not specified in the publication | Streptomyces albulus | |
| 0.078 | - |
S-adenosyl-L-methionine | isoform ClA1, at pH 7.8 and 37°C | uncultured bacterium | |
| 0.208 | - |
5-deoxy-5'-chloroadenosine | pH not specified in the publication, temperature not specified in the publication | Streptomyces albulus | |
| 0.208 | - |
5-deoxy-5-chloroadenosine | isoform ClA1, at pH 7.8 and 37°C | uncultured bacterium | |
| 0.248 | - |
5-deoxy-5'-chloroadenosine | pH not specified in the publication, temperature not specified in the publication | Umezawaea tangerina | |
| 0.249 | - |
5-deoxy-5-chloroadenosine | isoform ClA2, at pH 7.8 and 37°C | uncultured bacterium | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
S-adenosyl-L-methionine | isoform ClA2, at pH 7.8 and 37°C | uncultured bacterium | |
| 4.502 | - |
S-adenosyl-L-methionine | pH not specified in the publication, temperature not specified in the publication | Umezawaea tangerina | |
| 4.968 | - |
S-adenosyl-L-methionine | pH not specified in the publication, temperature not specified in the publication | Streptomyces albulus | |
| 4.97 | - |
S-adenosyl-L-methionine | isoform ClA1, at pH 7.8 and 37°C | uncultured bacterium | |
| 14.89 | - |
5-deoxy-5'-chloroadenosine | pH not specified in the publication, temperature not specified in the publication | Streptomyces albulus | |
| 14.89 | - |
5-deoxy-5-chloroadenosine | isoform ClA1, at pH 7.8 and 37°C | uncultured bacterium | |
| 26.29 | - |
5-deoxy-5'-chloroadenosine | pH not specified in the publication, temperature not specified in the publication | Umezawaea tangerina | |
| 26.29 | - |
5-deoxy-5-chloroadenosine | isoform ClA2, at pH 7.8 and 37°C | uncultured bacterium | |
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