Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.94 extracted from

  • Eustaquio, A.S.; Pojer, F.; Noel, J.P.; Moore, B.S.
    Discovery and characterization of a marine bacterial SAM-dependent chlorinase (2008), Nat. Chem. Biol., 4, 69-74.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.94

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salinispora tropica CNB-440

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 28% (w/v) PEG 3350, 0.3 M KCl, 2 mM dithiothreitol, 0.1 M MOPSO, pH 7.0 Salinispora tropica CNB-440

Protein Variants

Protein Variants Comment Organism
G131S the mutation leads to the complete loss of all SalL halogenase activities and the inability to crystallize SalL Salinispora tropica CNB-440
W129F the mutant shows 52% chlorinase activity, 76% brominase activity, and 3% iodidase activity compared to the wild type enzyme Salinispora tropica CNB-440
Y70T the mutation results in a two-orders-of-magnitude reduction of SalL activity, the mutant shows 0.07% chlorinase activity, 0.1% brominase activity, and 0.4% iodidase activity compared to the wild type enzyme Salinispora tropica CNB-440
Y70T/G131S the mutant shows 0.3% chlorinase activity, 0.5% brominase activity, and no iodidase activity compared to the wild type enzyme Salinispora tropica CNB-440

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 5'-chloro-5'-deoxyadenosine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.001
-
 S-adenosyl-L-methionine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.0046
-
 5'-deoxy-5'-iodoadenosine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
45
-
 chloride in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
150
-
 bromide in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
260
-
 Iodide in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 3 * 30000, in solution as determined by sedimentation velocity studies Salinispora tropica CNB-440

Organism

Organism UniProt Comment Textmining
Salinispora tropica CNB-440 A4X3Q0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salinispora tropica CNB-440

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-deoxy-5'-chloroadenosine + L-methionine
-
 Salinispora tropica CNB-440 S-adenosyl-L-methionine + chloride
-
 r
additional information the enzyme does not accept fluoride and 5-deoxy-5'-fluoroadenosine as substrates Salinispora tropica CNB-440 ?
-
 ?
S-adenosyl-L-methionine + bromide the enzyme also accepts bromide as substrate (with 90% relative activity compared to chloride) but the brominase activity is probably not biologically relevant in this marine bacterium Salinispora tropica CNB-440 5'-deoxy-5'-bromoadenosine + L-methionine
-
 r
S-adenosyl-L-methionine + chloride
-
 Salinispora tropica CNB-440 5'-deoxy-5'-chloroadenosine + L-methionine
-
 r
S-adenosyl-L-methionine + iodide the enzyme also accepts iodide as substrate (with 33% relative activity compared to chloride) but the iodinase activity is probably not biologically relevant in this marine bacterium Salinispora tropica CNB-440 5'-deoxy-5'-iodoadenosine + L-methionine
-
 r

Subunits

Subunits Comment Organism
homotrimer 3 * 30000, in solution as determined by sedimentation velocity studies Salinispora tropica CNB-440

Synonyms

Synonyms Comment Organism
chlorinase
-
 Salinispora tropica CNB-440
SalL
-
 Salinispora tropica CNB-440

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 Iodide in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.013
-
 bromide in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.015
-
 S-adenosyl-L-methionine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.015
-
 chloride in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.2
-
 L-methionine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.2
-
 5'-chloro-5'-deoxyadenosine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.83
-
 5'-deoxy-5'-iodoadenosine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440

General Information

General Information Comment Organism
physiological function SalL functions in an orthogonal manner to biological chlorination reactions to initiate the biosynthesis of a new halogenated polyketide synthase building block Salinispora tropica CNB-440

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0000192
-
 Iodide in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.0000883
-
 bromide in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.0003
-
 chloride in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
0.077
-
 L-methionine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
15
-
 S-adenosyl-L-methionine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
181.1
-
 5'-deoxy-5'-iodoadenosine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440
1000
-
 5'-chloro-5'-deoxyadenosine in 50 mM phosphate buffer (pH 7.9) at 37°C Salinispora tropica CNB-440